GeneTex
United States (US)

Cdh2 antibody

GTX125962_40954_20151008_IHC-Wm_Z_1_18121410_348.jpg
GTX125962_40954_WB_Z_18121410_594.jpg
GTX125962_40954_20151008_IHC-Wm_Z_2_18121410_168.jpg

Cat No. GTX125962

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Wm
Reactivity Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC-Wm 1:50-1:500
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Positive Control

zebrafish brain

Predict Reactivity

Human, Mouse, Rat, Bovine, Dog, Chicken, Japanese Medaka(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of zebrafish Cdh2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cadherin 2, Type 1, N-Cadherin (Neuronal),Bib,Glo,Lyr,Ncad,Pac,Wu:Fb47H04,Cdh2

Cellular Localization

Cell membrane

Background

Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. N-cadherin may be involved in neuronal recognition mechanism. Controls neural tube morphogenesis. Required to allow mid- and hindbrain neurons to reach and maintain their appropriate positions within the neural tube by restricting neuronal motility within the embryonic mid- and hindbrain.

Database

Research Area

DATA IMAGES
GTX125962_40954_20151008_IHC-Wm_Z_1_18121410_348.jpg

GTX125962 IHC-Wm Image

Cdh2 antibody detects Cdh2 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Cdh2 antibody (GTX125962) dilution: 1:50.

GTX125962_40954_WB_Z_18121410_594.jpg

GTX125962 WB Image

Sample (30 µg of whole cell lysate)
A: zebrafish brain
7.5% SDS PAGE
GTX125962 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX125962_40954_20151008_IHC-Wm_Z_2_18121410_168.jpg

GTX125962 IHC-Wm Image

Cdh2 antibody detects Cdh2 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Cdh2 antibody (GTX125962) dilution: 1:50.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 389
$ 199