IHC-P:0.5-1.0ug/ml for 30 minutes at RT, FACS:0.5-1ug/million cells in 0.1ml.
Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This MAb reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.
Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.
Ab purified from Bioreactor Concentrate by PEG precipitation
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
St6 Beta-Galactoside Alpha-2,6-Sialyltransferase 1,Siat1,St6Gali,St6N,St6Gal1
Golgi apparatus , Golgi stack membrane; Single-pass type II membrane protein , Secreted
This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Three transcript variants encoding two different isoforms have been described. [provided by RefSeq, Aug 2009]
GTX34584 FACS Image
Surface staining of human lymphocytes with CDw75 Monoclonal Antibody (LN-1) (red) and isotype control (green). PPI negative population analyzed.
GTX34584 IHC-P Image
Formalin-fixed, paraffin-embedded normal human Spleen stained with CDw75 Monoclonal Antibody (LN-1).