GeneTex
United States (US)

Cdw75 antibody [LN-1]

GTX34584_20170712_FACS_18121410_581.jpg
GTX34584_20170712_IHC-P_18121410_982.jpg

Cat No. GTX34584

Host Mouse
Clonality Monoclonal
Clone Name LN-1
Isotype IgM
Application IHC-P, FACS
Reactivity Human
APPLICATION

Application Note

IHC-P:0.5-1.0ug/ml for 30 minutes at RT, FACS:0.5-1ug/million cells in 0.1ml.

Calculated MW

47 kDa. ( Note )

Specificity/Sensitivity

Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This MAb reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.

Purification

Ab purified from Bioreactor Concentrate by PEG precipitation

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

St6 Beta-Galactoside Alpha-2,6-Sialyltransferase 1,Siat1,St6Gali,St6N,St6Gal1

Cellular Localization

Golgi apparatus , Golgi stack membrane; Single-pass type II membrane protein , Secreted

Background

This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Three transcript variants encoding two different isoforms have been described. [provided by RefSeq, Aug 2009]

Database

DATA IMAGES
GTX34584_20170712_FACS_18121410_581.jpg

GTX34584 FACS Image

Surface staining of human lymphocytes with CDw75 Monoclonal Antibody (LN-1) (red) and isotype control (green). PPI negative population analyzed.

GTX34584_20170712_IHC-P_18121410_982.jpg

GTX34584 IHC-P Image

Formalin-fixed, paraffin-embedded normal human Spleen stained with CDw75 Monoclonal Antibody (LN-1).

Package List Price ($)
$ 329