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Cdw75 antibody [LN-1]

Anti-Cdw75 antibody [LN-1] used in IHC (Paraffin sections) (IHC-P). GTX34584
Anti-Cdw75 antibody [LN-1] used in Flow cytometry (FACS). GTX34584
Anti-Cdw75 antibody [LN-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34584

Cat No. GTX34584

Host

Mouse

Clonality

Monoclonal

Clone Name

LN-1

Isotype

IgM

Application

ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Immunogen

Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.

Purification

PEG precipitation

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SIAT1,ST6 betagalactoside alpha2,6sialyltransferase 1,ST6GAL1,ST6GalI,ST6N,Cdw75,ST6 beta-galactoside alpha-2,6-sialyltransferase 1

Cellular Localization

Golgi apparatus,Golgi stack membrane,Secreted

Background

This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017]

Database

Research Area

DATA IMAGES

Anti-Cdw75 antibody [LN-1] used in IHC (Paraffin sections) (IHC-P). GTX34584

GTX34584 IHC-P Image

IHC-P analysis of normal human spleen tissue using GTX34584 Cdw75 antibody [LN-1].

Anti-Cdw75 antibody [LN-1] used in Flow cytometry (FACS). GTX34584

GTX34584 FACS Image

FACS analysis of human lymphocytes using GTX34584 Cdw75 antibody [LN-1].
Red : Primary antibody
Green : Isotype control

Anti-Cdw75 antibody [LN-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34584

GTX34584 ICC/IF Image

ICC/IF analysis of MeOH-fixed HeLa cells using GTX34584 Cdw75 antibody [LN-1].
Green : Primary antibody
Red : nucleus

REFERENCE

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REVIEW

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