GeneTex
United States (US)

Centaurin alpha 1 antibody [C3], C-term

GTX106419_39897_20181115_ICC_IF_R_19011519_460.jpg
GTX106419_39897_IHC_19011506_212.jpg
GTX106419_WB_19011506_277.jpg

Cat No. GTX106419

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Centaurin alpha 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CENTA1 antibody, GCS1L antibody, p42IP4 antibody, ADAP1 antibody, cnt-a1 antibody, putative MAPK-activating protein PM25 antibody, arf-GAP with dual PH domain-containing protein 1 antibody, centaurin-alpha-1 antibody, "centaurin, alpha 1 antibody", centaurin-alpha antibody, ArfGAP with dual PH domains 1 antibody

Cellular Localization

Nucleus , Cytoplasm

Background

GTPase-activating protein for the ADP ribosylation factor family (Probable). Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4).
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Database

DATA IMAGES
GTX106419_39897_20181115_ICC_IF_R_19011519_460.jpg

GTX106419 ICC/IF Image

Centaurin alpha 1 antibody [C3], C-term detects Centaurin alpha 1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Centaurin alpha 1 stained by Centaurin alpha 1 antibody [C3], C-term (GTX106419) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX106419_39897_IHC_19011506_212.jpg

GTX106419 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric tissue, using Centaurin alpha 1(GTX106419) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106419_WB_19011506_277.jpg

GTX106419 WB Image

Sample(30 μg of whole cell lysate)
A: A431 (GTX27909)
B: HeLaS3
7.5% SDS PAGE
GTX106419 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169