APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji
Predict Reactivity
Mouse, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.44mg/ml
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Centaurin alpha 1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
CENTA1 antibody, GCS1L antibody, p42IP4 antibody, ADAP1 antibody, cnt-a1 antibody, putative MAPK-activating protein PM25 antibody, arf-GAP with dual PH domain-containing protein 1 antibody, centaurin-alpha-1 antibody, "centaurin, alpha 1 antibody", centaurin-alpha antibody, ArfGAP with dual PH domains 1 antibody
Cellular Localization
Nucleus , Cytoplasm
Background
GTPase-activating protein for the ADP ribosylation factor family (Probable). Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4).
Database
DATA IMAGES
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GTX106419 ICC/IF Image
Centaurin alpha 1 antibody [C3], C-term detects Centaurin alpha 1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Centaurin alpha 1 stained by Centaurin alpha 1 antibody [C3], C-term (GTX106419) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX106419 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human gastric tissue, using Centaurin alpha 1(GTX106419) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX106419 WB Image
Sample(30 μg of whole cell lysate)
A: A431 (GTX27909)
B: HeLaS3
7.5% SDS PAGE
GTX106419 diluted at 1:500
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