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Centaurin alpha 1 antibody [C3], C-term

Anti-Centaurin alpha 1 antibody [C3], C-term used in Western Blot (WB). GTX106419
Anti-Centaurin alpha 1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106419
Anti-Centaurin alpha 1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106419

Cat No. GTX106419

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($329),
25 μl ($169)
See all Centaurin alpha 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Centaurin alpha 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949962

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ArfGAP with dual PH domains 1 , CENTA1 , GCS1L , p42IP4

Cellular Localization

Nucleus , Cytoplasm

Background

GTPase-activating protein for the ADP ribosylation factor family (Probable). Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4).

Database

Research Area

DATA IMAGES

Anti-Centaurin alpha 1 antibody [C3], C-term used in Western Blot (WB). GTX106419

GTX106419 WB Image

Sample(30 μg of whole cell lysate)
A: A431 (GTX27909)
B: HeLaS3
7.5% SDS PAGE
GTX106419 diluted at 1:500

Anti-Centaurin alpha 1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106419

GTX106419 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric tissue, using Centaurin alpha 1(GTX106419) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Centaurin alpha 1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106419

GTX106419 ICC/IF Image

Centaurin alpha 1 antibody [C3], C-term detects Centaurin alpha 1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Centaurin alpha 1 stained by Centaurin alpha 1 antibody [C3], C-term (GTX106419) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169