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Centrin 1 antibody

Anti-Centrin 1 antibody used in Western Blot (WB). GTX00891
Anti-Centrin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00891

Cat No. GTX00891

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat, Hamster, Xenopus
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB ~1:1000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide conjugated to KLH, with a Cysteine residue linker corresponding to C terminal amino acids 155-168 (C-GEVNEEEFLRIMKK) of Human centrin-1

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

centrin 1 , CEN1 , CETN , CETN1 , Centrin 1

Cellular Localization

Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome

Background

The protein encoded by this gene plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. This protein is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES

Anti-Centrin 1 antibody used in Western Blot (WB). GTX00891

GTX00891 WB Image

WB analysis of various samples using GTX00891 Centrin 1 antibody.
Lane 1 : HeLa whole cell lysate (20 μg)
Lane 2 : MCF-7 whole cell lysate (20 μg)
Lane3 : NIH-3T3 whole cell lysate (20 μg)
Lane 4 : Xenopus eggs (50 μg)
Dilution : 1:1000

Anti-Centrin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00891

GTX00891 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00891 Centrin 1 antibody.
Fixation : 4% PFA
Permeabilization : 0.25% Triton X-100
Red : Primary antibody
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf