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Centrin 1 antibody

Anti-Centrin 1 antibody used in Western Blot (WB). GTX11257
Anti-Centrin 1 antibody used in Western Blot (WB). GTX11257
Anti-Centrin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11257
Anti-Centrin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11257

Cat. No. GTX11257

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Centrin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2,000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic peptide corresponding to the C-terminus of human centrin 1 (amino acids 157-172). The sequence is highly conserved in centrin 1 of many species, e.g. mouse, Drosophila, and Xenopus. It is also found in human and mouse centrin 2, but is not found in centrin 3.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

centrin 1 , CEN1 , CETN

Background

This protein plays an important role in the determination of centrosome position and segregation, and in the process of microtubule severing. This encoded protein is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.

Database

Research Area

DATA IMAGES

Anti-Centrin 1 antibody used in Western Blot (WB). GTX11257

GTX11257 WB Image

WB analysis of HEK-293T cells overexpressing human centrin-1 using GTX11257 Centrin 1 antibody.
Lane 1 : Antibody 0.3 μg/mL
Lane 2 : Antibody 0.3 μg/mL + centrin1 immunizing peptide (human, 157-172)

Anti-Centrin 1 antibody used in Western Blot (WB). GTX11257

GTX11257 WB Image

WB analysis of (Lane 1 and 2) human HT-29 ,( Lane 3 and 4) HEK-293T, (Lane 5 and 6) HeLa cells using GTX11257 Centrin 1 antibody.
(Lane 1,3,5 ) Antibody 0.3 μg/mL
(Lane 2,4,6) Antibody 0.3 μg/mL + centrin1 immunizing peptide

Anti-Centrin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11257

GTX11257 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX11257 Centrin 1 antibody at 6 μg/mL(green) with DAPI(blue).

Anti-Centrin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11257

GTX11257 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX11257 Centrin 1 antibody at 6 μg/mL (green) with DAPI (blue).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399