Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin 2 antibody (GTX129709) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin 2 antibody (GTX129709) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Centrin 2 antibody detects Centrin 2 protein by western blot analysis. Non-transfected (-) and CETN2-transfected (+, including Flag-tag) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Centrin 2 antibody (GTX129709) diluted by 1:20000.