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Centrin 3 antibody

Anti-Centrin 3 antibody used in Western Blot (WB). GTX114317
Anti-Centrin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX114317
Anti-Centrin 3 antibody used in Western Blot (WB). GTX114317
Anti-Centrin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114317

Cat. No. GTX114317

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

A549 , H1299 , HCT-116 , Neuro2A , CD830 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2

Predict Reactivity

Zebrafish, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Centrin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885338

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

centrin 3 , CDC31 , CEN3

Cellular Localization

Centrosome

Background

The protein encoded by this gene contains four EF-hand calcium binding domains, and is a member of the centrin protein family. Centrins are evolutionarily conserved proteins similar to the CDC31 protein of S. cerevisiae. Yeast CDC31 is located at the centrosome of interphase and mitotic cells, where it plays a fundamental role in centrosome duplication and separation. Multiple forms of the proteins similar to the yeast centrin have been identified in human and other mammalian cells, some of which have been shown to be associated with centrosome fractions. This protein appears to be one of the most abundant centrins associated with centrosome, which suggests a similar function to its yeast counterpart. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Centrin 3 antibody used in Western Blot (WB). GTX114317

GTX114317 WB Image

Centrin3 antibody detects Centrin3 protein by western blot analysis. Various whole cell extracts of mouse and rat (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin3 antibody (GTX114317) diluted by 1:1000.

Anti-Centrin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX114317

GTX114317 IHC-P Image

Centrin3 antibody detects Centrin3 protein at cytosol on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
Centrin3 antibody (GTX114317) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Centrin 3 antibody used in Western Blot (WB). GTX114317

GTX114317 WB Image

Centrin3 antibody detects Centrin3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin3 antibody (GTX114317) diluted by 1:1000.

Anti-Centrin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114317

GTX114317 ICC/IF Image

Centrin3 antibody detects Centrin3 protein at centrosome by immunofluorescent analysis.
Sample: A549 cells were fixed in 100% MeOH for 5 min.
Green: Centrin3 protein stained by Centrin3 antibody (GTX114317) diluted at 1:1000.
Red: alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169