APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , H1299 , HCT-116 , Neuro2A , CD830 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2
Predict Reactivity
Zebrafish, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Centrin 3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885338
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
centrin 3 , CDC31 , CEN3
Cellular Localization
Centrosome
Background
The protein encoded by this gene contains four EF-hand calcium binding domains, and is a member of the centrin protein family. Centrins are evolutionarily conserved proteins similar to the CDC31 protein of S. cerevisiae. Yeast CDC31 is located at the centrosome of interphase and mitotic cells, where it plays a fundamental role in centrosome duplication and separation. Multiple forms of the proteins similar to the yeast centrin have been identified in human and other mammalian cells, some of which have been shown to be associated with centrosome fractions. This protein appears to be one of the most abundant centrins associated with centrosome, which suggests a similar function to its yeast counterpart. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX114317 WB Image
Centrin3 antibody detects Centrin3 protein by western blot analysis. Various whole cell extracts of mouse and rat (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin3 antibody (GTX114317) diluted by 1:1000.
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GTX114317 IHC-P Image
Centrin3 antibody detects Centrin3 protein at cytosol on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
Centrin3 antibody (GTX114317) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX114317 WB Image
Centrin3 antibody detects Centrin3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin3 antibody (GTX114317) diluted by 1:1000.
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GTX114317 ICC/IF Image
Centrin3 antibody detects Centrin3 protein at centrosome by immunofluorescent analysis.
Sample: A549 cells were fixed in 100% MeOH for 5 min.
Green: Centrin3 protein stained by Centrin3 antibody (GTX114317) diluted at 1:1000.
Red: alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:500.
Blue: Hoechst 33342 staining.
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REFERENCE
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REVIEW
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