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Ceruloplasmin antibody [N1N2], N-term

Anti-Ceruloplasmin antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113168
Anti-Ceruloplasmin antibody [N1N2], N-term used in Western Blot (WB). GTX113168
Anti-Ceruloplasmin antibody [N1N2], N-term used in Western Blot (WB). GTX113168
Anti-Ceruloplasmin antibody [N1N2], N-term used in Western Blot (WB). GTX113168
Anti-Ceruloplasmin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113168

Cat. No. GTX113168

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Ceruloplasmin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Positive Control

human plasma , mouse plasma , rat plasma

Predict Reactivity

Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Ceruloplasmin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950024

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ceruloplasmin , CP-2

Cellular Localization

Secreted

Background

The protein encoded by this gene is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe(II)transferrin to Fe(III) transferrin. Mutations in this gene cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Ceruloplasmin antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113168

GTX113168 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Ceruloplasmin(GTX113168) antibody at 1:200 dilution.

Anti-Ceruloplasmin antibody [N1N2], N-term used in Western Blot (WB). GTX113168

GTX113168 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Ceruloplasmin antibody [N1N2], N-term (GTX113168) diluted at 1:1000.

Anti-Ceruloplasmin antibody [N1N2], N-term used in Western Blot (WB). GTX113168

GTX113168 WB Image

Ceruloplasmin antibody detects Ceruloplasmin protein by western blot analysis. Human tissue extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Ceruloplasmin antibody (GTX113168) diluted at 1:1000.

Anti-Ceruloplasmin antibody [N1N2], N-term used in Western Blot (WB). GTX113168

GTX113168 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Ceruloplasmin antibody [N1N2], N-term (GTX113168) diluted at 1:1000.

Anti-Ceruloplasmin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113168

GTX113168 IHC-P Image

Ceruloplasmin antibody [N1N2], N-term detects Ceruloplasmin protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Ceruloplasmin antibody [N1N2], N-term (GTX113168) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Wang Q et al. J Atheroscler Thromb 2016; (Epub) Iron Together with Lipid Downregulates Protein Levels of Ceruloplasmin in Macrophages Associated with Rapid Foam Cell Formation.
Application : WB
Reactivity : Mouse
Brandi J et al. J Proteomics 2016; 136 : 43112 Secretome protein signature of human pancreatic cancer stem-like cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169