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Chikungunya virus E1 antibody

Anti-Chikungunya virus E1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135187
Anti-Chikungunya virus E1 antibody used in Western Blot (WB). GTX135187
Anti-Chikungunya virus E1 antibody used in Western Blot (WB). GTX135187

Cat. No. GTX135187

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Chikungunya Virus
Package
100 μl ($399),
25 μl ($169)
See all Chikungunya virus E1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000
ICC/IF Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Chikungunya Virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Chikungunya virus E1 (Chikungunya virus (strain S27-African prototype)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887462

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Chikungunya virus E1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135187

GTX135187 ICC/IF Image

Immunofluorescent analysis of mock and Chikungunya virus-infected cells using Chikungunya virus nsP2 antibody [HL1488] (GTX135187).
Sample: Chikungunya virus non-infected and infected cells slide.
Green: Chikungunya virus nsP2 antibody [HL1488] (GTX135187) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Chikungunya virus E1 antibody used in Western Blot (WB). GTX135187

GTX135187 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chikungunya virus E1 antibody (GTX135187) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Chikungunya virus E1 antibody used in Western Blot (WB). GTX135187

GTX135187 WB Image

Chikungunya viral lysate (0.5 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Chikungunya virus E1 antibody (GTX135187) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Singh S et al. Biomolecules 2021; 11 (5) Povidone-Iodine Attenuates Viral Replication in Ocular Cells: Implications for Ocular Transmission of RNA Viruses.
Application : ICC/IF
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169