Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Chikungunya virus E3 antibody [HL2476] (GTX638825) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Chikungunya virus E3 antibody [HL2476] detects Chikungunya virus E3 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Chikungunya virus E3 stained by Chikungunya virus E3 antibody [HL2476] (GTX638825) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).