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Chikungunya virus nsP3 antibody [HL2479]

Recombinant antibody
Anti-Chikungunya virus nsP3 antibody [HL2479] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX638828
Anti-Chikungunya virus nsP3 antibody [HL2479] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638828
Anti-Chikungunya virus nsP3 antibody [HL2479] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638828
Anti-Chikungunya virus nsP3 antibody [HL2479] used in Western Blot (WB). GTX638828

Cat. No. GTX638828

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2479

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P (cell pellet)

Reactivity

 Chikungunya Virus
Package
100 μl ($399),
25 μl ($169)
See all Chikungunya virus nsP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:50000
ICC/IF Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Chikungunya Virus

Immunogen

Recombinant fragment of Chikungunya virus nsP3. (strain S27-African prototype)

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

DATA IMAGES

Anti-Chikungunya virus nsP3 antibody [HL2479] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX638828

GTX638828 IHC-P (cell pellet) Image

Chikungunya virus nsP3 antibody [HL2479] detects Chikungunya virus nsP3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mock and Chikungunya virus nsP3 - transfected 293T cell pellet.
Green: Chikungunya virus nsP3 stained by Chikungunya virus nsP3 antibody [HL2479] (GTX638828) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Chikungunya virus nsP3 antibody [HL2479] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638828

GTX638828 ICC/IF Image

Immunofluorescent analysis of Chikungunya virus infected cells using Chikungunya virus nsP3 antibody [HL2479] antibody (GTX638828).
Sample: Multiple virus infected cells slide.
Green: Chikungunya virus nsP3 antibody [HL2479] antibody (GTX638828) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Chikungunya virus nsP3 antibody [HL2479] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638828

GTX638828 ICC/IF Image

Chikungunya virus nsP3 antibody [HL2479] detects Chikungunya virus nsP3 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: Chikungunya virus nsP3 stained by Chikungunya virus nsP3 antibody [HL2479] (GTX638828) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-Chikungunya virus nsP3 antibody [HL2479] used in Western Blot (WB). GTX638828

GTX638828 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (5 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chikungunya virus nsP3 antibody [HL2479] (GTX638828) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations