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Chk1 antibody [2G1D5]

Anti-Chk1 antibody [2G1D5] used in Western Blot (WB). GTX83385
Anti-Chk1 antibody [2G1D5] used in Western Blot (WB). GTX83385

Cat. No. GTX83385

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2G1D5

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Chk1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF Assay dependent
ELISA 1/10000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of CHK1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

checkpoint kinase 1 , CHK1

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Chk1 antibody [2G1D5] used in Western Blot (WB). GTX83385

GTX83385 WB Image

WB analysis of A431 (1), HeLa (2), NIH3T3 (3) and K562 (4) cell lysate using GTX83385 Chk1 antibody [2G1D5].

Anti-Chk1 antibody [2G1D5] used in Western Blot (WB). GTX83385

GTX83385 WB Image

WB analysis of HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY CHK1 cDNA (2) using GTX83385 Chk1 antibody [2G1D5].

REFERENCE

Application Reference

Zhou L et al. Mol Cancer Res 2022; 20 (3) : 456-467 Chk1 Inhibition Potently Blocks STAT3 Tyrosine705 Phosphorylation, DNA-Binding Activity, and Activation of Downstream Targets in Human Multiple Myeloma Cells.
Application : ICC/IF
Reactivity : Human
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399