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Chk1 antibody [6F5]

Anti-Chk1 antibody [6F5] used in Western Blot (WB). GTX70303
Anti-Chk1 antibody [6F5] used in Western Blot (WB). GTX70303
Anti-Chk1 antibody [6F5] used in Western Blot (WB). GTX70303
Anti-Chk1 antibody [6F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70303

Cat No. GTX70303

Host

Mouse

Clonality

Monoclonal

Clone Name

6F5

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Product Note

Human Chk1

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length human Chk1

Purification

Protein G affinity purified
From hybridoma culture supernatant

Conjugation

Unconjugated

RRID

AB_377598

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

checkpoint kinase 1 , CHK1

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Chk1 antibody [6F5] used in Western Blot (WB). GTX70303

GTX70303 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk1 antibody [6F5] (GTX70303) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Chk1 antibody [6F5] used in Western Blot (WB). GTX70303

GTX70303 WB Image

Detection of human Chk1 protein in HeLa whole cell lysate using GeneTex Chk 1 6F5 monoclonal antibody GTX70303

Anti-Chk1 antibody [6F5] used in Western Blot (WB). GTX70303

GTX70303 WB Image

Chk1 antibody [6F5] detects Chk1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg A375 whole cell lysate/extract
10 % SDS-PAGE
Chk1 antibody [6F5] (GTX70303) dilution: 1:500

Anti-Chk1 antibody [6F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70303

GTX70303 ICC/IF Image

Chk1 antibody [6F5] detects Chk1 protein at centrosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Chk1 stained by Chk1 antibody [6F5] (GTX70303) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329