GeneTex
United States (US)

Chk1 antibody [6F5]

Chk1-antibody-6F5-GTX70303-WB-1_18121409_836.jpg
GTX70303_41099_WB_18121411_986.jpg
Chk1-antibody-6F5-GTX70303-WB-2_18121409_278.jpg

Cat No. GTX70303

Host Mouse
Clonality Monoclonal
Clone Name 6F5
Isotype IgG1
Application WB
Reactivity Human
APPLICATION

Application Note

For WB: Use at an assay dependent dilution. Predicted molecular weight: 52 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

54 kDa. ( Note )

Specificity/Sensitivity

Human Chk1
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length human Chk1

Purification

Protein G affinity purified
From hybridoma culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Checkpoint Kinase 1,Chk1,Chek1

Cellular Localization

Nuclear

Background

Chk1 is a kinase involved in the DNA damage checkpoint response, and can phosphorylate Cdc25C. It is hypothesized that Chk1 induces serine 216 phosphorylation of Cdc25C and subsequent binding negatively regulated Cdc25C, thus preventing it from activating Cdc2.

Database

Research Area

DATA IMAGES
Chk1-antibody-6F5-GTX70303-WB-1_18121409_836.jpg

GTX70303 WB Image

Detection of human Chk1 protein in HeLa whole cell lysate using GeneTex Chk 1 6F5 monoclonal antibody GTX70303

GTX70303_41099_WB_18121411_986.jpg

GTX70303 WB Image

Chk1 antibody [6F5] detects Chk1 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg HeLa whole cell lysate/extract
C. 30 µg A375 whole cell lysate/extract
10 % SDS-PAGE
Chk1 antibody [6F5] (GTX70303) dilution: 1:500

Chk1-antibody-6F5-GTX70303-WB-2_18121409_278.jpg

GTX70303 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
10% SDS PAGE
GTX70303 diluted at 1:500

SDS
PBS.pdf
Package List Price ($)
$ 319