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Chk1 antibody [C1C2-6], Internal

Anti-Chk1 antibody [C1C2-6], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100070
Anti-Chk1 antibody [C1C2-6], Internal used in IHC (Paraffin sections) (IHC-P). GTX100070
Anti-Chk1 antibody [C1C2-6], Internal used in Western Blot (WB). GTX100070

Cat No. GTX100070

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

human Chk1-transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Chk1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

checkpoint kinase 1,CHK1

Background

Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.

Database

Research Area

DATA IMAGES
Anti-Chk1 antibody [C1C2-6], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100070

GTX100070 ICC/IF Image

CHK1 antibody [C1C2-6], Internal detects Chk1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Chk1 protein stained by CHK1 antibody [C1C2-6], Internal (GTX100070) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-Chk1 antibody [C1C2-6], Internal used in IHC (Paraffin sections) (IHC-P). GTX100070

GTX100070 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCM-1 xenograft, using Chk1(GTX100070) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Chk1 antibody [C1C2-6], Internal used in Western Blot (WB). GTX100070

GTX100070 WB Image

Chk1 antibody [C1C2-6], Internal detects Chk1 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of human Chk1-transfected 293T cells
10 % SDS-PAGE
Chk1 antibody [C1C2-6], Internal (GTX100070) dilution: 1:5000

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf