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Chk1 antibody [DCS-310]

Anti-Chk1 antibody [DCS-310] used in (). GTX42610

Cat. No. GTX42610

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 DCS-310

Isotype

 IgG3

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Chk1 products

APPLICATION

Application Note

IHC-P: Use at a concentration of 1 μg/ml. Heat treatment is neccessary for paraffin embedded sections. Heat in a microwave oven, twice for 10 minutes in citrate buffer, pH 6.5.IP: Use at a concentration of 2 μg per 0.2 ml of cell extract. WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa). ECL detection is used.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length protein (Human).

Purification

Protein A affinity purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

checkpoint kinase 1 , CHK1

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Chk1 antibody [DCS-310] used in (). GTX42610

GTX42610 Image

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399