GeneTex
  • Country / Location Selection

United States (US)

Chk1 antibody

Anti-Chk1 antibody used in Western Blot (WB). GTX22845
Anti-Chk1 antibody used in Proximity ligation assay (PLA). GTX22845
Anti-Chk1 antibody used in Western Blot (WB). GTX22845

Cat No. GTX22845

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, PLA

Reactivity

Human
Package
50 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2500 - 1:15000
PLA 1:1000 - 1:10000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate pH7.0-8.0

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen this antibody was made to, maps to a region between residues 200 and 250 of human Checkpoint Kinase 1 using the numbering given in entry NP_001265.1 (GeneID 1111).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

checkpoint kinase 1 , CHK1

Cellular Localization

Nucleus,Cytoplasm

Background

Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.

Database

Research Area

DATA IMAGES

Anti-Chk1 antibody used in Western Blot (WB). GTX22845

GTX22845 WB Image

WB analysis of Jurkat and 293T cell lysate (in NETN lysis buffer) using GTX22845 Chk1 antibody.
Dilution : 0.4 μg/ml

Anti-Chk1 antibody used in Proximity ligation assay (PLA). GTX22845

GTX22845 PLA Image

PLA analysis of HeLa cells using GTX22845 Chk1 antibody and anti-human PCNA antibody.
Green : Actin
Red : positive signal
Blue : DAPI

Anti-Chk1 antibody used in Western Blot (WB). GTX22845

GTX22845 WB Image

WB analysis of HeLa cells treated with 10 Gy ionizing radiation (IR) or untreated using GTX22845 Chk1 antibody.
Dilution : 0.1 μg/ml

REFERENCE

There are currently no references for Chk1 antibody (GTX22845). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Chk1 antibody (GTX22845). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 329