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Chk1 (phospho Ser280) antibody

Anti-Chk1 (phospho Ser280) antibody used in Western Blot (WB). GTX50200
Anti-Chk1 (phospho Ser280) antibody used in Western Blot (WB). GTX50200

Cat No. GTX50200

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 280 (V-T-S(p)-G-G) derived from human Chk1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

checkpoint kinase 1 , CHK1

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Chk1 (phospho Ser280) antibody used in Western Blot (WB). GTX50200

GTX50200 WB Image

WB analysis of extracts from A2780 cells treated with calf intestinal phosphatase (CIP) using GTX50200 Chk1 (phospho Ser280) antibody.

Anti-Chk1 (phospho Ser280) antibody used in Western Blot (WB). GTX50200

GTX50200 WB Image

WB analysis of extracts from HepG2 cells untreated or treated with UV using GTX50200 Chk1 (phospho Ser280) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf