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Chk1 (phospho Ser345) antibody [C1C2], Internal

Anti-Chk1 (phospho Ser345) antibody [C1C2], Internal used in Western Blot (WB). GTX100065
Anti-Chk1 (phospho Ser345) antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100065
Anti-Chk1 (phospho Ser345) antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100065
Anti-Chk1 (phospho Ser345) antibody [C1C2], Internal used in Western Blot (WB). GTX100065
Anti-Chk1 (phospho Ser345) antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100065

Cat No. GTX100065

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HCT116 , HCT116 cells with 30uM cisplatin treatment for 24hr

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human Chk1 (phospho Ser345). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

checkpoint kinase 1,CHK1

Cellular Localization

Nucleus , Cytoplasm

Background

Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.

Database

Research Area

DATA IMAGES
Anti-Chk1 (phospho Ser345) antibody [C1C2], Internal used in Western Blot (WB). GTX100065

GTX100065 WB Image

Sample (30 µg of whole cell lysate)
A: HCT116 cells with mock treatment for 24 hr
B: HCT116 cells with 30 µM cisplatin treatment for 24 hr
10% SDS PAGE
GTX100065 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Chk1 (phospho Ser345) antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100065

GTX100065 ICC/IF Image

Chk1 (phospho Ser345) antibody [C1C2], Internal detects Chk1 (phospho Ser345) protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 100 J/m2 UVC and recover for 8 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Chk1 (phospho Ser345) protein stained by Chk1 (phospho Ser345) antibody [C1C2], Internal (GTX100065) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Chk1 (phospho Ser345) antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100065

GTX100065 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using Chk1 (phospho Ser345) (GTX100065) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Chk1 (phospho Ser345) antibody [C1C2], Internal used in Western Blot (WB). GTX100065

GTX100065 WB Image

Sample: 20 µg of HCT116 P53 whole cell lysate 10% SDS PAGE
Chk1-phopho-S345 antibody
GTX100065 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Chk1 (phospho Ser345) antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100065

GTX100065 IHC-P Image

Chk1 (phospho Ser345) antibody [C1C2], Internal detects Chk1 (phospho Ser345) protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Chk1 (phospho Ser345) antibody [C1C2], Internal (GTX100065) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Phosphorylated.pdf