Chk1 (phospho Ser345) antibody

Cat. No. GTX79111

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	IHC-P
Reactivity	Human
Package	100 μg ($399)

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Datasheet

View product citations for antibody GTX79111 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
IHC-P	1:50 - 1:100

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 345 (S-F-Sp-Q-P).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11165313

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CHEK1,CHK1,checkpoint kinase 1,Chk1

Cellular Localization

Nucleus,Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome

Background

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Gene ID: 1111 CHEK1
UniProt: O14757 CHEK1

Research Area

DATA IMAGES

GTX79111 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX79111 Chk1 (phospho Ser345) antibody.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 399

Qty