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Chk1 (phospho Ser345) antibody [HL122]

Recombinant antibody Orthogonal
Anti-Chk1 (phospho Ser345) antibody [HL122] used in Western Blot (WB). GTX635572
Anti-Chk1 (phospho Ser345) antibody [HL122] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635572
Anti-Chk1 (phospho Ser345) antibody [HL122] used in Western Blot (WB). GTX635572

Cat. No. GTX635572

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL122

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Chk1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser345 of human Chk1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888538

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

checkpoint kinase 1 , CHK1

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Chk1 (phospho Ser345) antibody [HL122] used in Western Blot (WB). GTX635572

GTX635572 WB Image

Orthogonal

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk1 (phosphpo Ser345) antibody [HL122] (GTX635572) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Chk1 (phospho Ser345) antibody [HL122] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635572

GTX635572 ICC/IF Image

Orthogonal

Chk1 (phosphpo Ser345) antibody [HL122] detects Chk1 (phosphpo Ser345) protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Chk1 (phosphpo Ser345) stained by Chk1 (phosphpo Ser345) antibody [HL122] (GTX635572) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Chk1 (phospho Ser345) antibody [HL122] used in Western Blot (WB). GTX635572

GTX635572 WB Image

Orthogonal

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk1 (phosphpo Ser345) antibody [HL122] (GTX635572) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations