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Chk2 antibody [2F4]

KO/KD Overexpression
Anti-Chk2 antibody [2F4] used in Western Blot (WB). GTX84685
Anti-Chk2 antibody [2F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84685
Anti-Chk2 antibody [2F4] used in Flow cytometry (FACS). GTX84685
Anti-Chk2 antibody [2F4] used in Western Blot (WB). GTX84685
Anti-Chk2 antibody [2F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84685
Anti-Chk2 antibody [2F4] used in Western Blot (WB). GTX84685
Anti-Chk2 antibody [2F4] used in Western Blot (WB). GTX84685
Anti-Chk2 antibody [2F4] used in Western Blot (WB). GTX84685

Cat. No. GTX84685

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2F4

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Rat, Dog, Monkey
Package
100 μl ($519)
See all Chk2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:50-1:100
FACS 1:100
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CHEK2 (NP_009125) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

checkpoint kinase 2 , CDS1 , CHK2 , HuCds1 , LFS2 , PP1425 , RAD53 , hCds1

Cellular Localization

nucleoplasm

Background

Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.

Database

Research Area

DATA IMAGES

Anti-Chk2 antibody [2F4] used in Western Blot (WB). GTX84685

GTX84685 WB Image

WB analysis of various cell lines using GTX84685 Chk2 antibody [2F4].
Loading : 35 ug per lane

Anti-Chk2 antibody [2F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84685

GTX84685 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX84685 Chk2 antibody [2F4].

Anti-Chk2 antibody [2F4] used in Flow cytometry (FACS). GTX84685

GTX84685 FACS Image

FACS analysis of HEK293T cells transfected with either Chk2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84685 Chk2 antibody [2F4].

Anti-Chk2 antibody [2F4] used in Western Blot (WB). GTX84685

GTX84685 WB Image

WB analysis of various samples using GTX84685 Chk2 antibody [2F4].
Loading : 10 ug per lane
Dilution : 1:200

Anti-Chk2 antibody [2F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84685

GTX84685 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Chk2 plasmid using GTX84685 Chk2 antibody [2F4].

Anti-Chk2 antibody [2F4] used in Western Blot (WB). GTX84685

GTX84685 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Chk2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84685 Chk2 antibody [2F4].
Loading : 5 ug per lane

Anti-Chk2 antibody [2F4] used in Western Blot (WB). GTX84685

GTX84685 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon) using GTX84685 Chk2 antibody [2F4].
Loading : 10 ug per lane
Dilution : 1:200

Anti-Chk2 antibody [2F4] used in Western Blot (WB). GTX84685

GTX84685 WB Image

KO/KD

WB analysis of wild type and Chk2 knock out HeLa cell lysate(10μg per lane) using GTX84685 Chk2 antibody [2F4].
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519