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Chk2 antibody [4B8]

Anti-Chk2 antibody [4B8] used in Western Blot (WB). GTX70100
Anti-Chk2 antibody [4B8] used in Immunoprecipitation (IP). GTX70100
Anti-Chk2 antibody [4B8] used in Western Blot (WB). GTX70100

Cat No. GTX70100

Host

Mouse

Clonality

Monoclonal

Clone Name

4B8

Isotype

IgG1

Application

WB, IP

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

293T , A431 , HepG2 , HeLa

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Checkpoint Kinase 2 , Cds1 , Chk2 , Hucds1 , Lfs2 , Pp1425 , Rad53 , Hcds1 , Chek2

Background

This antibody binds to Chk2. In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. [from RefSeq]

Database

Research Area

DATA IMAGES

Anti-Chk2 antibody [4B8] used in Western Blot (WB). GTX70100

GTX70100 WB Image

Wild-type (WT) and Chk2 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 antibody [4B8] (GTX70100) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Chk2 antibody [4B8] used in Immunoprecipitation (IP). GTX70100

GTX70100 IP Image

Immunoprecipitation of Chk2 protein from 293T whole cell extracts using 5 μg of Chk2 antibody (GTX70100).
Western blot analysis was performed using Chk2 antibody (GTX70100).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Chk2 antibody [4B8] used in Western Blot (WB). GTX70100

GTX70100 WB Image

CHEK2 antibody detects CHEK2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CHEK2 antibody (GTX70100) diluted at 1:500.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329