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Chk2 antibody [5C4]

Anti-Chk2 antibody [5C4] used in Western Blot (WB). GTX84691
Anti-Chk2 antibody [5C4] used in Immunoprecipitation (IP). GTX84691
Anti-Chk2 antibody [5C4] used in Western Blot (WB). GTX84691
Anti-Chk2 antibody [5C4] used in IHC (Paraffin sections) (IHC-P). GTX84691
Anti-Chk2 antibody [5C4] used in IHC (Paraffin sections) (IHC-P). GTX84691
Anti-Chk2 antibody [5C4] used in IHC (Paraffin sections) (IHC-P). GTX84691
Anti-Chk2 antibody [5C4] used in IHC (Paraffin sections) (IHC-P). GTX84691
Anti-Chk2 antibody [5C4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84691
Anti-Chk2 antibody [5C4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84691
Anti-Chk2 antibody [5C4] used in IHC (Paraffin sections) (IHC-P). GTX84691
Anti-Chk2 antibody [5C4] used in Flow cytometry (FACS). GTX84691
Anti-Chk2 antibody [5C4] used in IHC (Paraffin sections) (IHC-P). GTX84691
Anti-Chk2 antibody [5C4] used in Western Blot (WB). GTX84691

Cat No. GTX84691

Host

Mouse

Clonality

Monoclonal

Clone Name

5C4

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, IP

Reactivity

Human, Dog
Package
100 μl ($409)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:50-1:100
IHC-P 1:150
FACS 1:100
IP 2ug/500ul
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CHEK2 (NP_009125) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

checkpoint kinase 2 , CDS1 , CHK2 , HuCds1 , LFS2 , PP1425 , RAD53 , hCds1

Cellular Localization

nucleoplasm

Background

Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.

Database

Research Area

DATA IMAGES

Anti-Chk2 antibody [5C4] used in Western Blot (WB). GTX84691

GTX84691 WB Image

WB analysis of HEK293T cells transfected with Chk2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84691 Chk2 antibody [5C4].
Loading : 5 ug per lane

Anti-Chk2 antibody [5C4] used in Immunoprecipitation (IP). GTX84691

GTX84691 IP Image

IP analysis of DDDDK tagged Chk2 overexpressed HEK293T lysate using GTX84691 Chk2 antibody [5C4].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-Chk2 antibody [5C4] used in Western Blot (WB). GTX84691

GTX84691 WB Image

WB analysis of various cell lines using GTX84691 Chk2 antibody [5C4].
Loading : 35 ug per lane

Anti-Chk2 antibody [5C4] used in IHC (Paraffin sections) (IHC-P). GTX84691

GTX84691 IHC-P Image

IHC-P analysis of human lung tissue using GTX84691 Chk2 antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Chk2 antibody [5C4] used in IHC (Paraffin sections) (IHC-P). GTX84691

GTX84691 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84691 Chk2 antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Chk2 antibody [5C4] used in IHC (Paraffin sections) (IHC-P). GTX84691

GTX84691 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84691 Chk2 antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Chk2 antibody [5C4] used in IHC (Paraffin sections) (IHC-P). GTX84691

GTX84691 IHC-P Image

IHC-P analysis of human breast tissue using GTX84691 Chk2 antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Chk2 antibody [5C4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84691

GTX84691 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Chk2 plasmid using GTX84691 Chk2 antibody [5C4].

Anti-Chk2 antibody [5C4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84691

GTX84691 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX84691 Chk2 antibody [5C4].

Anti-Chk2 antibody [5C4] used in IHC (Paraffin sections) (IHC-P). GTX84691

GTX84691 IHC-P Image

IHC-P analysis of human liver tissue using GTX84691 Chk2 antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Chk2 antibody [5C4] used in Flow cytometry (FACS). GTX84691

GTX84691 FACS Image

FACS analysis of Jurkat cells using GTX84691 Chk2 antibody [5C4].
Red : Primary antibody
Blue : Negative control antibody

Anti-Chk2 antibody [5C4] used in IHC (Paraffin sections) (IHC-P). GTX84691

GTX84691 IHC-P Image

IHC-P analysis of human colon tissue using GTX84691 Chk2 antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Chk2 antibody [5C4] used in Western Blot (WB). GTX84691

GTX84691 WB Image

WB analysis of wild type and Chk2 knock out HeLa cell lysate(10μg per lane) using GTX84691 Chk2 antibody [5C4].
Dilution : 1:500

REFERENCE

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REVIEW

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