Chk2 antibody [5C4]

Name: Chk2 antibody [5C4]
Brand: GeneTex
SKU: GTX84691
Price: 519.00 USD

Anti-Chk2 antibody [5C4] used in Western Blot (WB). GTX84691

Anti-Chk2 antibody [5C4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84691

Anti-Chk2 antibody [5C4] used in IHC (Paraffin sections) (IHC-P). GTX84691

Anti-Chk2 antibody [5C4] used in Immunoprecipitation (IP). GTX84691

Anti-Chk2 antibody [5C4] used in Flow cytometry (FACS). GTX84691

Cat. No. GTX84691

Host	Mouse
Clonality	Monoclonal
Clone Name	5C4
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, IP
Reactivity	Human, Dog
Package	100 μl ($519)

See all Chk2 products

Datasheet
Document

View product citations for antibody GTX84691 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
ICC/IF	1:50-1:100
IHC-P	1:150
FACS	1:100
IP	2ug/500ul

Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CHEK2 (NP_009125) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

checkpoint kinase 2 , CDS1 , CHK2 , HuCds1 , LFS2 , PP1425 , RAD53 , hCds1

Cellular Localization

nucleoplasm

Background

Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.

Database

Gene ID: 11200 CHEK2
UniProt: O96017 CHEK2

Research Area

DATA IMAGES

GTX84691 WB Image

WB analysis of HEK293T cells transfected with Chk2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84691 Chk2 antibody [5C4].
Loading : 5 ug per lane

GTX84691 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX84691 Chk2 antibody [5C4].

GTX84691 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Chk2 plasmid using GTX84691 Chk2 antibody [5C4].

GTX84691 WB Image

WB analysis of wild type and Chk2 knock out HeLa cell lysate(10μg per lane) using GTX84691 Chk2 antibody [5C4].
Dilution : 1:500

GTX84691 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84691 Chk2 antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84691 IHC-P Image

IHC-P analysis of human lung tissue using GTX84691 Chk2 antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84691 IHC-P Image

IHC-P analysis of human liver tissue using GTX84691 Chk2 antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84691 IP Image

IP analysis of DDDDK tagged Chk2 overexpressed HEK293T lysate using GTX84691 Chk2 antibody [5C4].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

GTX84691 IHC-P Image

IHC-P analysis of human breast tissue using GTX84691 Chk2 antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84691 WB Image

WB analysis of various cell lines using GTX84691 Chk2 antibody [5C4].
Loading : 35 ug per lane

GTX84691 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84691 Chk2 antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84691 IHC-P Image

IHC-P analysis of human colon tissue using GTX84691 Chk2 antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84691 FACS Image

FACS analysis of Jurkat cells using GTX84691 Chk2 antibody [5C4].
Red : Primary antibody
Blue : Negative control antibody

REFERENCE

There are currently no references for Chk2 antibody [5C4] (GTX84691). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for Chk2 antibody [5C4] (GTX84691). Be the first to share your experience with this product.

Submit a Review

SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

Chk2 antibody [5C4]

Cat. No. GTX84691

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84691 WB Image

GTX84691 ICC/IF Image

GTX84691 ICC/IF Image

GTX84691 WB Image

GTX84691 IHC-P Image

GTX84691 IHC-P Image

GTX84691 IHC-P Image

GTX84691 IP Image

GTX84691 IHC-P Image

GTX84691 WB Image

GTX84691 IHC-P Image

GTX84691 IHC-P Image

GTX84691 FACS Image

REFERENCE

REVIEW

Product

Research

Support

Company

© 1998-2022 GeneTex, Inc. All rights reserved.

All products are for research use only—Not for use in diagnostic or therapeutic applications.