FACS analysis of Jurkat cells using GTX84691 Chk2 antibody [5C4].
Red : Primary antibody
Blue : Negative control antibody

IHC-P analysis of human colon tissue using GTX84691 Chk2 antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human kidney tissue using GTX84691 Chk2 antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

WB analysis of various cell lines using GTX84691 Chk2 antibody [5C4].
Loading : 35 ug per lane

IHC-P analysis of human breast tissue using GTX84691 Chk2 antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IP analysis of DDDDK tagged Chk2 overexpressed HEK293T lysate using GTX84691 Chk2 antibody [5C4].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

IHC-P analysis of human liver tissue using GTX84691 Chk2 antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human lung tissue using GTX84691 Chk2 antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human pancreas tissue using GTX84691 Chk2 antibody [5C4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

WB analysis of wild type and Chk2 knock out HeLa cell lysate(10μg per lane) using GTX84691 Chk2 antibody [5C4].
Dilution : 1:500

ICC/IF analysis of COS7 cells transiently transfected with Chk2 plasmid using GTX84691 Chk2 antibody [5C4].

ICC/IF analysis of HT29 cells using GTX84691 Chk2 antibody [5C4].

WB analysis of HEK293T cells transfected with Chk2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84691 Chk2 antibody [5C4].
Loading : 5 ug per lane