GeneTex
United States (US)

Chk2 antibody [8F12]

GTX70295_42310_20151210_WB_18121411_863.jpg
CHK2-antibody-8F12-GTX70295-ICC_IF-1_18121409_673.jpg

Cat No. GTX70295

Host Mouse
Clonality Monoclonal
Clone Name 8F12
Isotype IgG1
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

~61 kDa. ( Note )

Positive Control

HepG2, MCF-7, or LN-Cap total cell extract

Specificity/Sensitivity

GTX70295 is specific for human CHK2 protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant CHK2 protein

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Checkpoint Kinase 2,Cds1,Chk2,Hucds1,Lfs2,Pp1425,Rad53,Hcds1,Chek2

Cellular Localization

Nuclear

Background

CHK2 controls cell cycle checkpoint and may participate in transduction of the DNA damage and replicational stress signals. CHK2 belongs to the Ser/Thr protein kinase family and its kinase actibity is up-regulated by autophosphorylation, It is phosphorylated rapidly in response to DNA damage and to replication block.

Database

Research Area

DATA IMAGES
GTX70295_42310_20151210_WB_18121411_863.jpg

GTX70295 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 antibody [8F12] (GTX70295) diluted at 1:500.

CHK2-antibody-8F12-GTX70295-ICC_IF-1_18121409_673.jpg

GTX70295 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using (GTX70295) antibody at 1:500 dilution.

REFERENCE
Package List Price ($)
$ 319