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Chk2 antibody [8F12]

Anti-Chk2 antibody [8F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70295
Anti-Chk2 antibody [8F12] used in Western Blot (WB). GTX70295
Anti-Chk2 antibody [8F12] used in Western Blot (WB). GTX70295

Cat No. GTX70295

Host

Mouse

Clonality

Monoclonal

Clone Name

8F12

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

~61 kDa. ( Note )

Positive Control

HepG2, MCF-7, or LN-Cap total cell extract

Specificity/Sensitivity

GTX70295 is specific for human CHK2 protein.

PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant CHK2 protein

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Checkpoint Kinase 2 , Cds1 , Chk2 , Hucds1 , Lfs2 , Pp1425 , Rad53 , Hcds1 , Chek2

Cellular Localization

Nuclear

Background

CHK2 controls cell cycle checkpoint and may participate in transduction of the DNA damage and replicational stress signals. CHK2 belongs to the Ser/Thr protein kinase family and its kinase actibity is up-regulated by autophosphorylation, It is phosphorylated rapidly in response to DNA damage and to replication block.

Database

Research Area

DATA IMAGES

Anti-Chk2 antibody [8F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70295

GTX70295 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using (GTX70295) antibody at 1:500 dilution.

Anti-Chk2 antibody [8F12] used in Western Blot (WB). GTX70295

GTX70295 WB Image

The WB analysis of Chk2 antibody [8F12] was published by Lee JH and colleagues in the journal PLoS One in 2014 . PMID: 24933654

Anti-Chk2 antibody [8F12] used in Western Blot (WB). GTX70295

GTX70295 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 antibody [8F12] (GTX70295) diluted at 1:500.

REFERENCE

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Package List Price ($)
$ 329