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Chk2 antibody [GT1148]

Recombinant antibody
Anti-Chk2 antibody [GT1148] used in Western Blot (WB). GTX00927
Anti-Chk2 antibody [GT1148] used in IHC (Paraffin sections) (IHC-P). GTX00927
Anti-Chk2 antibody [GT1148] used in Western Blot (WB). GTX00927

Cat No. GTX00927

Host

Rabbit

Clonality

Monoclonal

Clone Name

GT1148

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.3) with 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifμge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from hmuan Chk2

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

checkpoint kinase 2 , CDS1 , CHK2 , HuCds1 , LFS2 , PP1425 , RAD53 , hCds1

Cellular Localization

nucleoplasm

Background

In response to DNA damage and replication blocks, cell cycle progression is halted throμgh the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thoμght to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES

Anti-Chk2 antibody [GT1148] used in Western Blot (WB). GTX00927

GTX00927 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 antibody [GT1148] (GTX00927) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Chk2 antibody [GT1148] used in IHC (Paraffin sections) (IHC-P). GTX00927

GTX00927 IHC-P Image

IHC-P analysis of hmuan colon tissue section using GTX00927 Chk2 antibody [GT1148].
Dlution : 1:100

Anti-Chk2 antibody [GT1148] used in Western Blot (WB). GTX00927

GTX00927 WB Image

WB analysis of Jurkat and HeLa whole cell lysates using GTX00927 Chk2 antibody [GT1148].
Dilution : 1:1000
Loading : 25μg

REFERENCE

There are currently no references for Chk2 antibody [GT1148] (GTX00927). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Chk2 antibody [GT1148] (GTX00927). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369