Wild-type (WT) and Chk2 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 antibody (GTX113055) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sample (30 ug of whole cell lysate)
A: Jurkat
7.5% SDS PAGE
GTX113055 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Chk2 (GTX113055) antibody at 1:500 dilution.