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Chk2 antibody

Anti-Chk2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28108
Anti-Chk2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28108
Anti-Chk2 antibody used in Western Blot (WB). GTX28108

Cat. No. GTX28108

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($479)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF 1-5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Product Note

This antibody recognizes an epitope at the N-terminus of Chk2 (Checkpoint Kinase 2).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to aa 1-50 of human Chk2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CDS1 , CHEK2 , CHK2 , HuCds1 , LFS2 , PP1425 , RAD53 , checkpoint kinase 2 , hCds1 , Chk2

Cellular Localization

Nucleus,PML body,Nucleoplasm

Background

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES

Anti-Chk2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28108

GTX28108 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX28108 Chk2 antibody.
Dilution : 1 μg/ml.

Anti-Chk2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28108

GTX28108 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX28108 Chk2 antibody.
Red: Primary antibody
Blue: DAPI

Anti-Chk2 antibody used in Western Blot (WB). GTX28108

GTX28108 WB Image

WB analysis of various samples using GTX28108 Chk2 antibody.
Land A : K562 whole cell lysateLane B : Jurkat whole cell lysate
Lane C : HL-60 whole cell lysate
Dilution : 1 μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 479