GeneTex
United States (US)

Chk2 antibody

Chk2-antibody__GTX28108-1_18121409_229.jpg

Cat No. GTX28108

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

Western blot at 0.5 to 1 μg/ml and a 60 kDa band can bedetected.

Calculated MW

61 kDa. ( Note )

Positive Control

Jurkat or K562 cell lysate.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: SRESDVEAQQSHGSSAC, corresponding to amino acids 2-18 of Human Chk2.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HuCds1, CHK2, 114480, PP1425, LFS2, RAD53, CDS1, O96017, 11200, CHEK2

Cellular Localization

Nuclear

Background

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2 (1), p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2(6), were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53 (7,8). Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer (9).

Database

Research Area

DATA IMAGES
Chk2-antibody__GTX28108-1_18121409_229.jpg

GTX28108 WB Image

Western blot analysis of Chk2 expression in K562 (A), Jurkat (B), and HL-60 (C) whole cell lysates with GTX28108

Package List Price ($)
$ 329