APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| 1 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat Cell Slide(GTX25533) , Jurkat Cell Lysate(GTX27899) , K562 Cell Lysate(GTX27911)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Chk2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2.The immunogen is located within the first 50 amino acids of Chk2.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
checkpoint kinase 2 , CDS1 , CHK2 , HuCds1 , LFS2 , PP1425 , RAD53 , hCds1
Cellular Localization
nucleoplasm
Background
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarc
Database
Research Area
DATA IMAGES
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GTX31659 WB Image
WB analysis of (A) K562, (B) Jurkat, and (C) HL-60 whole cell lysates using GTX31659 Chk2 antibody.
Working concentration : 1 μg/ml
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GTX31659 ICC/IF Image
ICC/IF analysis of Jurkat cells using GTX31659 Chk2 antibody.
Working concentration : 5 μg/ml
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GTX31659 ICC/IF Image
ICC/IF analysis of Jurkat cells using GTX31659 Chk2 antibody.
Working concentration : 1 μg/ml
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REFERENCE
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REVIEW
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