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Chk2 antibody

Anti-Chk2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31659
Anti-Chk2 antibody used in Western Blot (WB). GTX31659
Anti-Chk2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31659

Cat No. GTX31659

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF 1 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

Jurkat Cell Slide(GTX25533) , Jurkat Cell Lysate(GTX27899) , K562 Cell Lysate(GTX27911)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Chk2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2.The immunogen is located within the first 50 amino acids of Chk2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

checkpoint kinase 2 , CDS1 , CHK2 , HuCds1 , LFS2 , PP1425 , RAD53 , hCds1

Cellular Localization

nucleoplasm

Background

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarc

Database

Research Area

DATA IMAGES

Anti-Chk2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31659

GTX31659 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX31659 Chk2 antibody.
Working concentration : 1 μg/ml

Anti-Chk2 antibody used in Western Blot (WB). GTX31659

GTX31659 WB Image

WB analysis of (A) K562, (B) Jurkat, and (C) HL-60 whole cell lysates using GTX31659 Chk2 antibody.
Working concentration : 1 μg/ml

Anti-Chk2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31659

GTX31659 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX31659 Chk2 antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339