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Chk2 (phospho Thr68) antibody

Anti-Chk2 (phospho Thr68) antibody used in Western Blot (WB). GTX132204
Anti-Chk2 (phospho Thr68) antibody used in Western Blot (WB). GTX132204
Anti-Chk2 (phospho Thr68) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132204

Cat No. GTX132204

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

HeLa , 293T , 293T (100μM etoposide for 2hr)

Predict Reactivity

Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues around human Chk2 (phospho Thr68). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

checkpoint kinase 2,CDS1,CHK2,HuCds1,LFS2,PP1425,RAD53,hCds1

Cellular Localization

Isoform 2: Nucleus , Isoform 4: Nucleus , Isoform 7: Nucleus , Isoform 9: Nucleus , Isoform 12: Nucleus

Background

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Chk2 (phospho Thr68) antibody used in Western Blot (WB). GTX132204

GTX132204 WB Image

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Thr68) antibody (GTX132204) diluted at 1:1000.

Anti-Chk2 (phospho Thr68) antibody used in Western Blot (WB). GTX132204

GTX132204 WB Image

Wild-type (WT) and Chk2 (phospho Thr68) knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Thr68) antibody (GTX132204) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Chk2 (phospho Thr68) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132204

GTX132204 ICC/IF Image

Chk2 (phospho Thr68) antibody detects Chk2 (phospho Thr68) protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Chk2 (phospho Thr68) stained by Chk2 (phospho Thr68) antibody (GTX132204) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Scale bar= 10 μm.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf