APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.03-0.3μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89623-PEP).
Product Note
This antibody is expected to recognise isoform 1 (NP_066264.3), isoform 2 (NP_065574.3) and isoform 3 (NP_001136405.1).
Predict Reactivity
Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence CKEKATRPSQGHQP, from the C Terminus of the protein sequence according to NP_066264.3; NP_065574.3; NP_001136405.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
choline O-acetyltransferase , CHOACTASE , CMS1A , CMS1A2 , CMS6
Background
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
Database
Research Area
DATA IMAGES
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GTX89623 WB Image
WB analysis of human placenta lysate using GTX89623 Choline Acetyltransferase antibody, C-term. Dilution : 0.03μg/ml Loading : 35μg protein in RIPA buffer
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REFERENCE
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REVIEW
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