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Choline Acetyltransferase antibody [HL2014]

Recombinant antibody Overexpression
Anti-Choline Acetyltransferase antibody [HL2014] used in Western Blot (WB). GTX637915
Anti-Choline Acetyltransferase antibody [HL2014] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637915
Anti-Choline Acetyltransferase antibody [HL2014] used in Western Blot (WB). GTX637915
Anti-Choline Acetyltransferase antibody [HL2014] used in IHC (Paraffin sections) (IHC-P). GTX637915
Anti-Choline Acetyltransferase antibody [HL2014] used in IHC (Paraffin sections) (IHC-P). GTX637915

Cat. No. GTX637915

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2014

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Choline Acetyltransferase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Choline Acetyltransferase. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

choline O-acetyltransferase , CHOACTASE , CMS1A , CMS1A2 , CMS6

Cellular Localization

Nucleus,Cytosol

Background

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-Choline Acetyltransferase antibody [HL2014] used in Western Blot (WB). GTX637915

GTX637915 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [HL2014] (GTX637915) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Choline Acetyltransferase antibody [HL2014] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637915

GTX637915 ICC/IF Image

Choline Acetyltransferase antibody [HL2014] detects Choline Acetyltransferase protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Choline Acetyltransferase stained by Choline Acetyltransferase antibody [HL2014] (GTX637915) diluted at 1:250.
Red: Tau, an Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Choline Acetyltransferase antibody [HL2014] used in Western Blot (WB). GTX637915

GTX637915 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [HL2014] (GTX637915) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Choline Acetyltransferase antibody [HL2014] used in IHC (Paraffin sections) (IHC-P). GTX637915

GTX637915 IHC-P Image

Choline Acetyltransferase antibody [HL2014] detects Choline Acetyltransferase protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: Choline Acetyltransferase stained by Choline Acetyltransferase antibody [HL2014] (GTX637915) diluted at 1:100.
Red: Cytoskeleton, a GTX631836 marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836_41855, 1, 500) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Choline Acetyltransferase antibody [HL2014] used in IHC (Paraffin sections) (IHC-P). GTX637915

GTX637915 IHC-P Image

Choline Acetyltransferase antibody [HL2014] detects Choline Acetyltransferase protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spinal cord.
Choline Acetyltransferase stained by Choline Acetyltransferase antibody [HL2014] (GTX637915) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations