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Chromogranin A antibody [CHGA/777]

Anti-Chromogranin A antibody [CHGA/777] used in IHC (Paraffin sections) (IHC-P). GTX34598
Anti-Chromogranin A antibody [CHGA/777] used in IHC (Paraffin sections) (IHC-P). GTX34598
Anti-Chromogranin A antibody [CHGA/777] used in Western Blot (WB). GTX34598
Anti-Chromogranin A antibody [CHGA/777] used in IHC (Paraffin sections) (IHC-P). GTX34598

Cat No. GTX34598

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 CHGA/777

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μg ($429)
See all Chromogranin A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Specificity/Sensitivity

Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant full-length human CHGA protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Chromogranin A , Cga , Chga

Background

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Database

Research Area

DATA IMAGES

Anti-Chromogranin A antibody [CHGA/777] used in IHC (Paraffin sections) (IHC-P). GTX34598

GTX34598 IHC-P Image

Formalin-fixed, paraffin-embedded human Adrenal Gland stained with Chromogranin A Monoclonal Antibody (CHGA/777)

Anti-Chromogranin A antibody [CHGA/777] used in IHC (Paraffin sections) (IHC-P). GTX34598

GTX34598 IHC-P Image

Formalin-paraffin human Pheochromocytoma stained with Chromogranin A MAb (CHGA/777)

Anti-Chromogranin A antibody [CHGA/777] used in Western Blot (WB). GTX34598

GTX34598 WB Image

Western Blot of human Panc-1 Cell Lysate Chromogranin A MAb (CHGA/777)

Anti-Chromogranin A antibody [CHGA/777] used in IHC (Paraffin sections) (IHC-P). GTX34598

GTX34598 IHC-P Image

Formalin-fixed, paraffin-embedded human Pancreas stained with Chromogranin A Monoclonal Antibody (CHGA/777)

REFERENCE

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REVIEW

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Package List Price ($)
$ 429