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Chromogranin A antibody [PHE5]

Anti-Chromogranin A antibody [PHE5] used in IHC (Paraffin sections) (IHC-P). GTX34605

Cat No. GTX34605

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 PHE5

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human, Monkey
Package
100 μg ($339)
See all Chromogranin A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Specificity/Sensitivity

We do not recommend use of this product for Mouse,Rat.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human pheochromocytoma

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CGA , CHGA , chromogranin A , Chromogranin A

Cellular Localization

Cytoplasmic vesicle,Secretory vesicle lumen,Secretory vesicle membrane,Secreted,Secretory vesicle

Background

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Database

Research Area

DATA IMAGES

Anti-Chromogranin A antibody [PHE5] used in IHC (Paraffin sections) (IHC-P). GTX34605

GTX34605 IHC-P Image

IHC-P analysis of human adrenal gland tissue using GTX34605 Chromogranin A antibody [PHE5].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339