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Chromogranin A antibody

Comparison Orthogonal
Anti-Chromogranin A antibody used in Western Blot (WB). GTX113165
Anti-Chromogranin A antibody used in IHC (Paraffin sections) (IHC-P). GTX113165

Cat. No. GTX113165

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Chromogranin A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

SH-SY-5Y conditioned medium

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Chromogranin A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036567

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromogranin A , CGA

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Chromogranin A antibody used in Western Blot (WB). GTX113165

GTX113165 WB Image

Comparison Orthogonal

SH-SY-5Y whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Chromogranin A antibody (GTX113165) diluted at 1:500 and competitor's antibody (sc-1488) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Chromogranin A antibody used in IHC (Paraffin sections) (IHC-P). GTX113165

GTX113165 IHC-P Image

Comparison

Chromogranin A antibody detects Chromogranin A protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Chromogranin A stained by Chromogranin A antibody (GTX113165) diluted at 1:2000 and competitor's antibody (sc-1488) diluted at 1:20.

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Wu SY et al. Autophagy 2023; 19 (4) : 1239-1257 Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo.
Application : WB
Reactivity : Mouse
Feiling Song et al. Front Cell Dev Biol 2021; 9 : 745412 An Active Fraction of Trillium tschonoskii Promotes the Regeneration of Intestinal Epithelial Cells After Irradiation.
Application : IHC-P
Reactivity : Mouse
Wang S et al. Theranostics 2020; 10 (22) : 10171-10185 Me6TREN targets β-catenin signaling to stimulate intestinal stem cell regeneration after radiation.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169