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Chromogranin A antibody

Comparison Orthogonal
Anti-Chromogranin A antibody used in Western Blot (WB). GTX113165
Anti-Chromogranin A antibody used in IHC (Paraffin sections) (IHC-P). GTX113165

Cat. No. GTX113165

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Chromogranin A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

SH-SY-5Y conditioned medium

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Chromogranin A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036567

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

chromogranin A , CGA

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Chromogranin A antibody used in Western Blot (WB). GTX113165

GTX113165 WB Image

Comparison Orthogonal

SH-SY-5Y whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Chromogranin A antibody (GTX113165) diluted at 1:500 and competitor's antibody (sc-1488) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Chromogranin A antibody used in IHC (Paraffin sections) (IHC-P). GTX113165

GTX113165 IHC-P Image

Comparison

Chromogranin A antibody detects Chromogranin A protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Chromogranin A stained by Chromogranin A antibody (GTX113165) diluted at 1:2000 and competitor's antibody (sc-1488) diluted at 1:20.

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Feiling Song et al. Front Cell Dev Biol 2021; 9 : 745412 An Active Fraction of Trillium tschonoskii Promotes the Regeneration of Intestinal Epithelial Cells After Irradiation.
Application : IHC-P
Reactivity : Mouse
Wang S et al. Theranostics 2020; 10 (22) : 10171-10185 Me6TREN targets β-catenin signaling to stimulate intestinal stem cell regeneration after radiation.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169