GeneTex
United States (US)

Chromogranin C antibody

GTX115049_40247_IHC_18121410_131.jpg
GTX115049_40247_IHC_R_18121410_706.jpg
GTX115049_WB_18121410_218.jpg
GTX115049_40247_IHC_M_18121410_831.jpg

Cat No. GTX115049

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

NT2D1 , IMR32 , U87-MG

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Chromogranin C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Secretogranin Ii,Chgc,Em66,Sn,Sgii,Scg2

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Studies in rodents suggest that the full-length protein, secretogranin II, is involved in the packaging or sorting of peptide hormones and neuropeptides into secretory vesicles. The full-length protein is cleaved to produce the active peptide secretoneurin, which exerts chemotaxic effects on specific cell types, and EM66, whose function is unknown. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115049_40247_IHC_18121410_131.jpg

GTX115049 IHC-P Image

Chromogranin C antibody detects SCG2 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Chromogranin C antibody (GTX115049) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115049_40247_IHC_R_18121410_706.jpg

GTX115049 IHC-P Image

Chromogranin C antibody detects SCG2 protein at cytosol on rat RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded rat RT2 xenograft.
Chromogranin C antibody (GTX115049) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115049_WB_18121410_218.jpg

GTX115049 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: IMR32
C: U87-MG
7.5% SDS PAGE
GTX115049 diluted at 1:1000

GTX115049_40247_IHC_M_18121410_831.jpg

GTX115049 IHC-P Image

Chromogranin C antibody detects SCG2 protein at cytosol on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain tissue.
Chromogranin C antibody (GTX115049) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf