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Chromogranin C antibody

Anti-Chromogranin C antibody used in IHC (Paraffin sections) (IHC-P). GTX115049
Anti-Chromogranin C antibody used in Western Blot (WB). GTX115049
Anti-Chromogranin C antibody used in IHC (Paraffin sections) (IHC-P). GTX115049
Anti-Chromogranin C antibody used in IHC (Paraffin sections) (IHC-P). GTX115049

Cat. No. GTX115049

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Chromogranin C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

NT2D1 , IMR32 , U87-MG

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Chromogranin C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620189

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

secretogranin II , CHGC , EM66 , SN , SgII

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Studies in rodents suggest that the full-length protein, secretogranin II, is involved in the packaging or sorting of peptide hormones and neuropeptides into secretory vesicles. The full-length protein is cleaved to produce the active peptide secretoneurin, which exerts chemotaxic effects on specific cell types, and EM66, whose function is unknown. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Chromogranin C antibody used in IHC (Paraffin sections) (IHC-P). GTX115049

GTX115049 IHC-P Image

Chromogranin C antibody detects SCG2 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Chromogranin C antibody (GTX115049) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Chromogranin C antibody used in Western Blot (WB). GTX115049

GTX115049 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: IMR32
C: U87-MG
7.5% SDS PAGE
GTX115049 diluted at 1:1000

Anti-Chromogranin C antibody used in IHC (Paraffin sections) (IHC-P). GTX115049

GTX115049 IHC-P Image

Chromogranin C antibody detects SCG2 protein at cytosol on rat RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded rat RT2 xenograft.
Chromogranin C antibody (GTX115049) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Chromogranin C antibody used in IHC (Paraffin sections) (IHC-P). GTX115049

GTX115049 IHC-P Image

Chromogranin C antibody detects SCG2 protein at cytosol on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain tissue.
Chromogranin C antibody (GTX115049) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169