Chromogranin C antibody

Name: Chromogranin C antibody
Brand: GeneTex
SKU: GTX116446
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-Chromogranin C antibody used in Western Blot (WB). GTX116446

Anti-Chromogranin C antibody used in IHC (Paraffin sections) (IHC-P). GTX116446

Cat. No. GTX116446

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Chromogranin C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

IMR32 , U87-MG , mouse brain , rat brain

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Chromogranin C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171949

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

secretogranin II , CHGC , EM66 , SN , SgII

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Studies in rodents suggest that the full-length protein, secretogranin II, is involved in the packaging or sorting of peptide hormones and neuropeptides into secretory vesicles. The full-length protein is cleaved to produce the active peptide secretoneurin, which exerts chemotaxic effects on specific cell types, and EM66, whose function is unknown. [provided by RefSeq]

Database

Gene ID: 7857 SCG2
UniProt: P13521 SCG2

Research Area

DATA IMAGES

GTX116446 WB Image

Chromogranin C antibody detects SCG2 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
7.5% SDS-PAGE
Chromogranin C antibody (GTX116446) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116446 IHC-P Image

Chromogranin C antibody detects SCG2 protein at cytosol on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
Chromogranin C antibody (GTX116446) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX116446 IHC-P Image

Chromogranin C antibody detects SCG2 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Chromogranin C antibody (GTX116446) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX116446 WB Image

Chromogranin C antibody detects SCG2 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
7.5% SDS-PAGE
Chromogranin C antibody (GTX116446) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116446 IHC-P Image

Chromogranin C antibody detects Chromogranin C protein at membrane on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Chromogranin C antibody (GTX116446) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX116446 WB Image

Sample (30 μg of whole cell lysate)
A: IMR32
B: U87-MG
7.5% SDS PAGE
GTX116446 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Lin Z et al. Adv Sci (Weinh) 2022; 9 (27) : e2202263 Tuning the Size of Large Dense-Core Vesicles and Quantal Neurotransmitter Release via Secretogranin II Liquid-Liquid Phase Separation.

Federico A et al. Mol Ther Oncolytics 2020; 18 : 83-99 Mithramycin A and Mithralog EC-8042 Inhibit SETDB1 Expression and Its Oncogenic Activity in Malignant Melanoma.

Xiang H et al. J Int Med Res 2020; 48 (9) : 300060520957541 Characterization of blood-derived exosomal proteins after exercise.

Application : WB

Reactivity : Mouse

Moro A et al. PLoS Biol 2020; 18 (8) : e3000826 CaMKII controls neuromodulation via neuropeptide gene expression and axonal targeting of neuropeptide vesicles.

Application : WB

Reactivity : Mouse

Valiya Veettil M et al. J Virol 2020; (Epub) Kaposi's Sarcoma Associated Herpesvirus Infection Induces the Expression of Neuroendocrine Genes in Endothelial Cells.

Application : WB

Reactivity : Human

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