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Citrate synthase antibody [GT1761]

KO/KD Orthogonal
Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143
Anti-Citrate synthase antibody [GT1761] used in IHC (Paraffin sections) (IHC-P). GTX628143
Anti-Citrate synthase antibody [GT1761] used in IHC (Paraffin sections) (IHC-P). GTX628143
Anti-Citrate synthase antibody [GT1761] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628143
Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143
Anti-Citrate synthase antibody [GT1761] used in IHC (Paraffin sections) (IHC-P). GTX628143
Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143
Anti-Citrate synthase antibody [GT1761] used in IHC (Paraffin sections) (IHC-P). GTX628143
Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143
Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143
Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143
Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143

Cat No. GTX628143

Reference 6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1761

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($379),
25 μl ($169)
See all Citrate synthase products
PRODUCT

Summary

Citrate synthase antibody detects citrate synthase, the 52 kDa enzyme that catalyzes the first reaction in the citric acid cycle (Krebs cycle) to convert acetyl-CoA and oxaloacetate to citrate and CoA-SH. Citrate synthase exists within the mitochondrial matrix in eukaryotic cells and is used commonly as a marker of intact mitochondria.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , mouse brain , rat brain , zebrafish brain , zebrafish heart

Predict Reactivity

Bovine, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Citrate synthetase. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888020

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

citrate synthase

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143

GTX628143 WB Image

Orthogonal

HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (GTX628143) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [GT1761] used in IHC (Paraffin sections) (IHC-P). GTX628143

GTX628143 IHC-P Image

Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Citrate synthase antibody [GT1761] used in IHC (Paraffin sections) (IHC-P). GTX628143

GTX628143 IHC-P Image

Citrate synthetase antibody detects CS protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
Citrate synthetase antibody (GTX628143) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Citrate synthase antibody [GT1761] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628143

GTX628143 ICC/IF Image

Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Citrate synthetase protein stained by Citrate synthetase antibody [GT1761] (GTX628143) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Blue: Hoechst 33342 staining.
Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143

GTX628143 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (GTX628143) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [GT1761] used in IHC (Paraffin sections) (IHC-P). GTX628143

GTX628143 IHC-P Image

Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143

GTX628143 WB Image

Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
12% SDS-PAGE
Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [GT1761] used in IHC (Paraffin sections) (IHC-P). GTX628143

GTX628143 IHC-P Image

Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143

GTX628143 WB Image

Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
12% SDS-PAGE
Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143

GTX628143 WB Image

Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis.
A. 30 μg zebrafish brain lysate/extract
10% SDS-PAGE
Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143

GTX628143 WB Image

Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis.
A. 30 μg zebrafish heart lysate/extract
10% SDS-PAGE
Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143

GTX628143 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (GTX628143) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Pablo Ranea-Robles et al. Cell Mol Life Sci 2021; 78 (14) : 5631-5646 Murine deficiency of peroxisomal L-bifunctional protein (EHHADH) causes medium-chain 3-hydroxydicarboxylic aciduria and perturbs hepatic cholesterol homeostasis.
Pablo Ranea-Robles et al. Kidney360 2021; 2 (9) : 1441-1454 Peroxisomal L-bifunctional protein (EHHADH) deficiency causes male-specific kidney hypertrophy and proximal tubular injury in mice.
Leandro J et al. Hum Mol Genet 2020; 29 (7) : 1168-1179 DHTKD1 and OGDH display substrate overlap in cultured cells and form a hybrid 2-oxo acid dehydrogenase complex in vivo.
Zhang X et al. J Biol Chem 2020; 295 (23) : 8078-8095 Structure-function analyses of the G729R 2-oxoadipate dehydrogenase genetic variant associated with a disorder of l-lysine metabolism.
Application : WB
Jo?o Leandro et al. bioRxiv 2019; (Epub) DHTKD1 and OGDH display in vivo substrate overlap and form a hybrid ketoacid dehydrogenase complex.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Package List Price ($)
$ 379
$ 169
Custom Conjugations