Summary
Citrate synthase antibody detects citrate synthase, the 52 kDa enzyme that catalyzes the first reaction in the citric acid cycle (Krebs cycle) to convert acetyl-CoA and oxaloacetate to citrate and CoA-SH. Citrate synthase exists within the mitochondrial matrix in eukaryotic cells and is used commonly as a marker of intact mitochondria.
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
zebrafish brain , 293T , A431 , HeLa , HepG2 , A375 , mouse brain , zebrafish heart , rat brain
Predict Reactivity
Bovine, Pig, Xenopus tropicalis(>80% identity)
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Citrate synthetase. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888020
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.