PRODUCT
Summary
Citrate synthase antibody detects citrate synthase, the 52 kDa enzyme that catalyzes the first reaction in the citric acid cycle (Krebs cycle) to convert acetyl-CoA and oxaloacetate to citrate and CoA-SH. Citrate synthase exists within the mitochondrial matrix in eukaryotic cells and is used commonly as a marker of intact mitochondria.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , A375 , mouse brain , rat brain , zebrafish brain , zebrafish heart
Predict Reactivity
Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Citrate synthetase. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888020
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
citrate synthase
Cellular Localization
Mitochondrion matrix
Background
The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX628143 WB Image
HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (GTX628143) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628143 IHC-P Image
Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX628143 IHC-P Image
Citrate synthetase antibody detects CS protein at cytosol on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. Citrate synthetase antibody (GTX628143) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX628143 ICC/IF Image
Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Citrate synthetase protein stained by Citrate synthetase antibody [GT1761] (GTX628143) diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.
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GTX628143 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (GTX628143) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628143 IHC-P Image
Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX628143 WB Image
Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis. A. 50 μg mouse brain lysate/extract 12% SDS-PAGE Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628143 IHC-P Image
Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX628143 WB Image
Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract E. 30 μg A375 whole cell lysate/extract 12% SDS-PAGE Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628143 WB Image
Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis. A. 30 μg zebrafish brain lysate/extract 10% SDS-PAGE Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628143 WB Image
Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis. A. 30 μg zebrafish heart lysate/extract 10% SDS-PAGE Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628143 WB Image
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (GTX628143) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
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