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Citrate synthase antibody [N2C3]

KO/KD
Anti-Citrate synthase antibody [N2C3] used in Immunoprecipitation (IP). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Whole mount) (IHC-Wm). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Whole mount) (IHC-Wm). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

Cat. No. GTX110624

Reference 23
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm, IP

Reactivity

 Human, Mouse, Rat, Zebrafish, Chicken, Monkey, Frog
Package
100 μl ($399),
25 μl ($169)
See all Citrate synthase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

mouse brain , rat brain , Rat muscle , 293T

Predict Reactivity

Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Citrate synthetase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950045

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

citrate synthase

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Citrate synthase antibody [N2C3] used in Immunoprecipitation (IP). GTX110624

GTX110624 IP Image

Immunoprecipitation of Citrate synthetase protein from 293T whole cell extracts using 5 μg of Citrate synthetase antibody [N2C3] (GTX110624).
Western blot analysis was performed using Citrate synthetase antibody [N2C3] (GTX110624).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

GTX110624 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Citrate synthetase antibody [N2C3] (GTX110624) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

GTX110624 WB Image

Citrate synthetase antibody detects Citrate synthetase protein by Western blot analysis. Rat tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody (GTX110624) at a dilution of 1:1000.

Anti-Citrate synthase antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110624

GTX110624 ICC/IF Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-Citrate synthase antibody [N2C3] used in IHC (Whole mount) (IHC-Wm). GTX110624

GTX110624 IHC-Wm Image

Citrate synthase antibody [N2C3] detects Citrate synthase protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Citrate synthase antibody [N2C3] (GTX110624) dilution: 1:50.
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

GTX110624 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [N2C3] used in IHC (Whole mount) (IHC-Wm). GTX110624

GTX110624 IHC-Wm Image

Citrate synthase antibody [N2C3] detects Citrate synthase protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green: Citrate synthase stained by Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:100.

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthase antibody [N2C3] detects Citrate synthase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Citrate synthase stained by Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

GTX110624 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cs antibody (GTX110624) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

GTX110624 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Todorova V et al. Mol Neurodegener 2023; 18 (1) : 15 Deficits in mitochondrial TCA cycle and OXPHOS precede rod photoreceptor degeneration during chronic HIF activation.
Demchenko IT et al. Front Mol Neurosci 2022; 15 : 1062410 GAT inhibition preserves cerebral blood flow and reduces oxidant damage to mitochondria in rodents exposed to extreme hyperbaric oxygen.
Todorova V et al. Sci Rep 2022; 12 (1) : 20195 Retinal Layer Separation (ReLayS) method enables the molecular analysis of photoreceptor segments and cell bodies, as well as the inner retina.
Katharina I Zittlau et al. Mol Cell Proteomics 2022; 21 (2) : 100191 Temporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-Dependent Mitophagy.
Application : WB, ICC/IF
Reactivity : Human
Hagir Suliman et al. Front Physiol 2021; 12 : 683098 Annexin A1 Tripeptide Mimetic Increases Sirtuin-3 and Augments Mitochondrial Function to Limit Ischemic Kidney Injury.
Application : IHC-P
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

Citrate synthase antibody [N2C3] Cat. No. GTX110624

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 3 users reviews)
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014. PMID: 25489948
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28515438
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28515438
Submit a Review
Application Reference
Todorova V et al. Mol Neurodegener 2023; 18 (1):15 Deficits in mitochondrial TCA cycle and OXPHOS precede rod photoreceptor degeneration during chronic HIF activation.
Demchenko IT et al. Front Mol Neurosci 2022; 15:1062410 GAT inhibition preserves cerebral blood flow and reduces oxidant damage to mitochondria in rodents exposed to extreme hyperbaric oxygen.
Todorova V et al. Sci Rep 2022; 12 (1):20195 Retinal Layer Separation (ReLayS) method enables the molecular analysis of photoreceptor segments and cell bodies, as well as the inner retina.
Katharina I Zittlau et al. Mol Cell Proteomics 2022; 21 (2):100191 Temporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-Dependent Mitophagy.
Application : WB, ICC/IF
Reactivity : Human
Hagir Suliman et al. Front Physiol 2021; 12:683098 Annexin A1 Tripeptide Mimetic Increases Sirtuin-3 and Augments Mitochondrial Function to Limit Ischemic Kidney Injury.
Application : IHC-P
Reactivity : Mouse
Leandro J et al. Hum Mol Genet 2020; 29 (7):1168-1179 DHTKD1 and OGDH display substrate overlap in cultured cells and form a hybrid 2-oxo acid dehydrogenase complex in vivo.
Ding CC et al. Nat Metab 2020; 2 (3):270-277 MESH1 is a cytosolic NADPH phosphatase that regulates ferroptosis.
Al-Attar R et al. Gene 2020; 761:145039 RAGE against the stress: Mitochondrial suppression in hypometabolic hearts.
Application : WB
Reactivity : Frog
Chao-Chieh Lin et al. bioRxiv 2020; (Epub) Adipose-Derived Stem Cells Modulate BV2 Microglial M1/M2 Polarization by Producing Glial Cell-Derived Neurotrophic Factor.
Application : WB
Reactivity : Human
Jo?o Leandro et al. bioRxiv 2019; (Epub) DHTKD1 and OGDH display in vivo substrate overlap and form a hybrid ketoacid dehydrogenase complex.
Application : WB
Reactivity : Human
Leandro J et al. Mol Genet Metab 2019; (Epub) Mild inborn errors of metabolism in commonly used inbred mouse strains.
Application : WB
Reactivity : Mouse
Ding C.C. et al. bioRxiv 2018; (Epub) Mammalian stringent-like response mediated by the cytosolic NADPH phosphatase MESH1.
Application : WB
Reactivity : Human
Nam M et al. Sci Rep 2017; 7 (1):2013 Mitochondrial retrograde signaling connects respiratory capacity to thermogenic gene expression.
Suliman HB et al. JCI Insight 2017; 2 (3):e89676 Mitochondrial quality-control dysregulation in conditional HO-1-/- mice.
Kim Y et al. Appl Physiol Nutr Metab 2017; (Epub) Effects of long-term exposures to low iron and branched-chain amino acid containing diets on aging skeletal muscle of Fisher 344 Brown Norway rats.
Application : WB
Reactivity : Rat
Lake NJ et al. Am J Hum Genet 2017; 101 (2):239-254 Biallelic Mutations in MRPS34 Lead to Instability of the Small Mitoribosomal Subunit and Leigh Syndrome.
Application : WB
Reactivity : Human
Baati N et al. Biochim Biophys Acta 2017; Myostatin deficiency is associated with lipidomic abnormalities in skeletal muscles.
Application : WB
Reactivity : Mouse
Sanchez I et al. Hum Mol Genet 2016; Ataxin-1 regulates the cerebellar bioenergetics proteome through the GSK3β-mTOR pathway which is altered in Spinocerebellar ataxia type 1 (SCA1).
Application : WB
Reactivity : Mouse
Fredenburgh LE et al. Am J Physiol Lung Cell Mol Physiol 2015; 309 (8):L834-46 Effects of inhaled CO administration on acute lung injury in baboons with pneumococcal pneumonia.
Application : WB
Reactivity : Monkey
Abdel Khalek W et al. PLoS One 2014; 9 (12):e114388 SIRT3, a Mitochondrial NAD+-Dependent Deacetylase, Is Involved in the Regulation of Myoblast Differentiation.
Application : WB
Reactivity : Mouse
Alan L. Chang et al. Free Radical Biology and Medicine 2014; Redox regulation of mitophagy in the lung during murine Staphylococcus aureus sepsis
Application : ICC/IF
Reactivity : Mouse
Mizushima S et al. Development 2014; 141 (19):3799-806 The birth of quail chicks after intracytoplasmic sperm injection.
Application : WB
Reactivity : Chicken
Rosenkilde M et al. Scand J Med Sci Sports 2013; Changes in peak fat oxidation in response to different doses of endurance training.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169