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Citrate synthase antibody [N2C3]

Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Immunoprecipitation (IP). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624
Anti-Citrate synthase antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110624

Cat No. GTX110624

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Chicken, Monkey, Frog
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , rat brain , Rat muscle

Predict Reactivity

Zebrafish, Bovine, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Citrate synthetase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950045

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

citrate synthase

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

GTX110624 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

GTX110624 WB Image

Citrate synthetase antibody detects Citrate synthetase protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody (GTX110624) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [N2C3] used in Immunoprecipitation (IP). GTX110624

GTX110624 IP Image

Immunoprecipitation of Citrate synthetase protein from 293T whole cell extracts using 5 μg of Citrate synthetase antibody [N2C3] (GTX110624).
Western blot analysis was performed using Citrate synthetase antibody [N2C3] (GTX110624).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Citrate synthase antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110624

GTX110624 ICC/IF Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Citrate synthetase protein stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

GTX110624 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Citrate synthetase antibody [N2C3] (GTX110624) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

GTX110624 WB Image

Citrate synthetase antibody detects Citrate synthetase protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody (GTX110624) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Citrate synthase antibody [N2C3] used in Western Blot (WB). GTX110624

GTX110624 WB Image

Citrate synthetase antibody detects Citrate synthetase protein by Western blot analysis. Rat tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody (GTX110624) at a dilution of 1:1000.

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Citrate synthase antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX110624

GTX110624 IHC-P Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Citrate synthase antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110624

GTX110624 ICC/IF Image

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

REFERENCE

REVIEW

Citrate synthase antibody [N2C3] Cat No. GTX110624

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 25489948
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28515438
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28515438
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf