IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent concentration. Predicted molecular weight: 192 kDa. Optimal dilutions/concentrations should be determined by the end user.
Recognizes the clathrin heavy chain from a diverse range of species. In nervous tissue it crossreacts with another coated vesicle polypeptide of 190kD which is not present in isolated clathrin triskelions. In some epithelial cells of certain species the antibody may crossreact with a cytokeratin component. It detects coated vesicles from different organs and tissues (e.g. brain, ovary, mammary gland).
Preservative: 0.1% Sodium Azide; Constituents: 1mg/ml BSA, PBS. pH 7.2
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
Coated vesicles (clathrin) of bovine brain
Ammonium sulfate precipitation followed by gel filtration.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Clathrin Heavy Chain,Cltc
Cell Membrane and Cytoplasmic
Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.