GeneTex
United States (US)

Clathrin Heavy chain antibody [2Q2166]

Cat No. GTX14401

Host Mouse
Clonality Monoclonal
Clone Name 2Q2166
Isotype IgM
Application WB, IHC-Fr
Reactivity Bovine
APPLICATION

Application Note

IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent concentration. Predicted molecular weight: 192 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

192 kDa. ( Note )

Specificity/Sensitivity

Recognizes the clathrin heavy chain from a diverse range of species. In nervous tissue it crossreacts with another coated vesicle polypeptide of 190kD which is not present in isolated clathrin triskelions. In some epithelial cells of certain species the antibody may crossreact with a cytokeratin component. It detects coated vesicles from different organs and tissues (e.g. brain, ovary, mammary gland).
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.1% Sodium Azide; Constituents: 1mg/ml BSA, PBS. pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Coated vesicles (clathrin) of bovine brain

Purification

Purified IgM
Ammonium sulfate precipitation followed by gel filtration.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Clathrin Heavy Chain,Cltc

Cellular Localization

Cell Membrane and Cytoplasmic

Background

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.

Database

Research Area

Package List Price ($)
$ 319