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Clathrin Heavy chain antibody [BF-06]

Anti-Clathrin Heavy chain antibody [BF-06] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80218
Anti-Clathrin Heavy chain antibody [BF-06] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80218

Cat. No. GTX80218

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 BF-06

Isotype

 IgM

Application

 WB, ICC/IF, FACS, IP, ELISA

Reactivity

 Human, Mouse, Rat, Bovine, Pig
Package
100 μg ($399)
See all Clathrin Heavy chain products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

192 kDa. ( Note )

Product Note

This antibody recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa protein involved in receptor-mediated endocytosis.

PROPERTIES

Form

Liquid

Buffer

TBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Purified by precipitation and chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CHC , CHC17 , CLH17 , CLTC , CLTCL2 , Hc , clathrin heavy chain , Clathrin heavy chain

Cellular Localization

Cytoplasmic vesicle membrane,Membrane,Coated pit,Melanosome,Cytoplasm,Cytoskeleton,Spindle

Background

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Clathrin Heavy chain antibody [BF-06] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80218

GTX80218 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX80218 Clathrin Heavy chain antibody [BF-06].
Green : Primary antibody
Red : Actin
Blue : DAPI

Anti-Clathrin Heavy chain antibody [BF-06] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80218

GTX80218 ICC/IF Image

ICC/IF analysis of human primary fibroblasts using GTX80218 Clathrin Heavy chain antibody [BF-06].
Green : Primary antibody
Red : Actin
Blue : DAPI

REFERENCE

Application Reference

Rivera JF et al. bioRxiv 2023; ATLAS: A rationally designed anterograde transsynaptic tracer.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399