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Clathrin Heavy chain antibody

Anti-Clathrin Heavy chain antibody used in Western Blot (WB). GTX132976
Anti-Clathrin Heavy chain antibody used in Western Blot (WB). GTX132976
Anti-Clathrin Heavy chain antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132976

Cat No. GTX132976

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

192 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR-32 , SK-N-AS
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Clathrin Heavy chain. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

clathrin heavy chain,CHC,CHC17,CLH-17,CLTCL2,Hc,MRD56

Background

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Clathrin Heavy chain antibody used in Western Blot (WB). GTX132976

GTX132976 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Clathrin Heavy chain antibody (GTX132976) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Clathrin Heavy chain antibody used in Western Blot (WB). GTX132976

GTX132976 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Clathrin Heavy chain antibody (GTX132976) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Clathrin Heavy chain antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132976

GTX132976 ICC/IF Image

Clathrin Heavy chain antibody detects Clathrin Heavy chain protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Clathrin Heavy chain stained by Clathrin Heavy chain antibody (GTX132976) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
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Package List Price ($)
$ 319
$ 169