Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Clathrin Heavy chain antibody (GTX132976) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Clathrin Heavy chain antibody (GTX132976) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Clathrin Heavy chain antibody detects Clathrin Heavy chain protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Clathrin Heavy chain stained by Clathrin Heavy chain antibody (GTX132976) diluted at 1:500.
Blue: Hoechst 33342 staining.