Clathrin Heavy chain antibody

Name: Clathrin Heavy chain antibody
Brand: GeneTex
SKU: GTX132976
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Clathrin Heavy chain antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132976

Anti-Clathrin Heavy chain antibody used in Western Blot (WB). GTX132976

Cat. No. GTX132976

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

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Datasheet
Document

View product citations for antibody GTX132976 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent

Not tested in other applications.

Calculated MW

192 kDa. ( Note )

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Clathrin Heavy chain. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886794

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

clathrin heavy chain , CHC , CHC17 , CLH-17 , CLTCL2 , Hc , MRD56

Background

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008]

Database

Gene ID: 1213 CLTC
UniProt: Q00610 CLTC

Research Area

DATA IMAGES

GTX132976 ICC/IF Image

Clathrin Heavy chain antibody detects Clathrin Heavy chain protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Clathrin Heavy chain stained by Clathrin Heavy chain antibody (GTX132976) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX132976 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Clathrin Heavy chain antibody (GTX132976) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX132976 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Clathrin Heavy chain antibody (GTX132976) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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