GeneTex
United States (US)

Clathrin Light chain antibody [3F133] (Functional Grade)

Cat No. GTX14409

Host Mouse
Clonality Monoclonal
Clone Name 3F133
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat, Zebrafish, Bovine, Xenopus laevis, Fish
APPLICATION

Application Note

Recommended Starting Dilutions:
ICC: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 1 - 2 μg/ml.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 28 kDa.
Optimal dilutions/concentrations should be determined by the end user.

Positive Control

Placenta

Specificity/Sensitivity

Reacts with both LCa and LCb.
PROPERTIES

Form

Liquid

Buffer

10mM PBS (pH 7.4), with no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Synthetic peptide: EEDPAAAFLAQQESEIAGIEND, corresponding to amino acids 23-44 of the conserved regions of Cow Clathrin Light Chain.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Cell Membrane and Cytoplasmic

Background

Various cell types express both forms of clathrin LC in different relative levels. LCs are randomly distributed on clathrin triskelion, resulting in four types of clathrin triskelion with different LC composition (3LCa, 2LCa 1LCb, 2LCb-1LCa, 3LCb). In most cells, the LCa form is dominant. In contrast, cells with a regulated secretory pathway contain more LCb than LCa, suggesting that LCb may play a role in the specialized clathrin functions in these cells. Neuronal cells, express splicing variants of both LCa and LCb. A distinguishing feature of the LCb light chain is its ability to be phosphorylated by a casein kinase, at a site which is not present in the LCa sequence.
Package List Price ($)
$ 329