10mM PBS (pH 7.4), with no preservatives
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Synthetic peptide: EEDPAAAFLAQQESEIAGIEND, corresponding to amino acids 23-44 of the conserved regions of Cow Clathrin Light Chain.
Protein A purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Cell Membrane and Cytoplasmic
Various cell types express both forms of clathrin LC in different relative levels. LCs are randomly distributed on clathrin triskelion, resulting in four types of clathrin triskelion with different LC composition (3LCa, 2LCa 1LCb, 2LCb-1LCa, 3LCb). In most cells, the LCa form is dominant. In contrast, cells with a regulated secretory pathway contain more LCb than LCa, suggesting that LCb may play a role in the specialized clathrin functions in these cells. Neuronal cells, express splicing variants of both LCa and LCb. A distinguishing feature of the LCb light chain is its ability to be phosphorylated by a casein kinase, at a site which is not present in the LCa sequence.