GeneTex

  • Country / Location Selection

United States (US)

Claudin 1 antibody

Orthogonal
Anti-Claudin 1 antibody used in Western Blot (WB). GTX54539
Anti-Claudin 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54539
Anti-Claudin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54539
Anti-Claudin 1 antibody used in Western Blot (WB). GTX54539

Cat. No. GTX54539

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC

Reactivity

 Human, Mouse, Rat, Rabbit, Bovine, Dog, Avian
Package
100 μg ($399)
See all Claudin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/mL
ICC/IF 15-20 μg/mL
IHC-P 1:10-1:100
IHC-Fr Assay dependent
IP Assay dependent
ELISA 0.1-1 μg/ml
IHC Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide derived from the C-terminus of the human/mouse Claudin-1 protein.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887859

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

claudin 1 , CLD1 , ILVASC , SEMP1

Cellular Localization

Cell membrane

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Claudin 1 antibody used in Western Blot (WB). GTX54539

GTX54539 WB Image

WB analysis of Rat liver(Lane 1), Rat kidney(Lane 2), MDCK cells(Lane 3), Caco-2 cells(Lane 4), Mouse hepatocytes(Lane 5) lysate using GTX54539 Claudin 1 antibody.

Anti-Claudin 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54539

GTX54539 IHC-Fr Image

IHC-Fr analysis of rat small intestine tissue using GTX54539 Claudin 1 antibody.

Anti-Claudin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54539

GTX54539 IHC-P Image

IHC-P analysis of human liver tissue using GTX54539 Claudin 1 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-Claudin 1 antibody used in Western Blot (WB). GTX54539

GTX54539 WB Image

Orthogonal

The data was published in the 2021 in Front Cell Infect Microbiol. PMID: 35071031

REFERENCE

Application Reference

Duan C et al. Front Cell Infect Microbiol 2023; 13 : 1190602 Fucose ameliorates the proinflammatory property of Fusobacterium nucleatum in colitis via altering its metabolism.
Wanchao Hu et al. Front Cell Infect Microbiol 2021; 11 : 743454 Diallyl Disulfide (DADS) Ameliorates Intestinal Candida albicans Infection by Modulating the Gut microbiota and Metabolites and Providing Intestinal Protection in Mice.
Application : WB
Reactivity : Mouse
Hu W et al. Microb Pathog 2021; 154 : 104826 Alterations in the gut microbiota and metabolic profiles coincide with intestinal damage in mice with a bloodborne Candida albicans infection.
Application : WB
Reactivity : Mouse
Amador C. V. et al. J Clin Exp Pathol 2017; 7 : 6 MUC1 and C-Met Affects Proliferation, Intercellular Junctions and Invasion in Two Head and Neck Carcinoma Cell Lines.
Application : WB, ICC/IF
Reactivity : Human
Li CH et al. Arch Toxicol 2016; (Epub) Cytoplasmic aryl hydrocarbon receptor regulates glycogen synthase kinase 3 beta, accelerates vimentin degradation, and suppresses epithelial-mesenchymal transition in non-small cell lung cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Claudin 1 antibody (GTX54539). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399