GeneTex

  • Country / Location Selection

United States (US)

Claudin 5 antibody

Anti-Claudin 5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00796
Anti-Claudin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX00796
Anti-Claudin 5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00796
Anti-Claudin 5 antibody used in Western Blot (WB). GTX00796

Cat. No. GTX00796

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Monkey
Package
100 μl ($399)
See all Claudin 5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Predict Reactivity

Rabbit, Chicken, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Claudin 5, corresponding to a region within C-terminal amino acids.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AWAL , BEC1 , CLDN5 , CPETRL1 , TMVCF , claudin 5 , Claudin 5

Cellular Localization

Cell junction,Tight junction,Cell membrane

Background

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018]

Database

Research Area

DATA IMAGES

Anti-Claudin 5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00796

GTX00796 ICC/IF Image

ICC/IF analysis of PFA-fixed MCF7 cells using GTX00796 Claudin 5 antibody.
Red : Primary antibody
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-Claudin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX00796

GTX00796 IHC-P Image

IHC-P analysis of human colon tissue using GTX00796 Claudin 5 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

Anti-Claudin 5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00796

GTX00796 ICC/IF Image

ICC/IF analysis of PFA-fixed A549 cells using GTX00796 Claudin 5 antibody.
Red : Primary antibody
Permeabilization : 0.1% saponin
Dilution : 1:400

Anti-Claudin 5 antibody used in Western Blot (WB). GTX00796

GTX00796 WB Image

WB analysis of VERO cell lysate using GTX00796 Claudin 5 antibody. The lane on the left was treated with blocking peptide.

REFERENCE

Application Reference

Ma Z et al. Cell Death Dis 2021; 12 (9) : 840 Tumor-derived exosomal miR-3157-3p promotes angiogenesis, vascular permeability and metastasis by targeting TIMP/KLF2 in non-small cell lung cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Claudin 5 antibody (GTX00796). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399