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Claudin 5 antibody

Anti-Claudin 5 antibody used in Western Blot (WB). GTX49370
Anti-Claudin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX49370
Anti-Claudin 5 antibody used in Western Blot (WB). GTX49370
Anti-Claudin 5 antibody used in Western Blot (WB). GTX49370

Cat No. GTX49370

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($349)

APPLICATION

Application Note

WB: 1:500-1:1000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 171-220 of Human Claudin-5.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Claudin 5 , Awal , Bec1 , Cpetrl1 , Tmdvcf , Tmvcf , Cldn5

Background

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2008]

Database

Research Area

DATA IMAGES

Anti-Claudin 5 antibody used in Western Blot (WB). GTX49370

GTX49370 WB Image

Western blot (WB) analysis of Claudin-5 pAb at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:COS-7 whole cell lysate(40ug) Lane4:Hela whole cell lysate(40ug)

Anti-Claudin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX49370

GTX49370 IHC-P Image

Immunohistochemistry (IHC) analyzes of Claudin-5 pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Anti-Claudin 5 antibody used in Western Blot (WB). GTX49370

GTX49370 WB Image

Western blot (WB) analysis of Claudin-5 pAb at 1:500 dilution Lane1:Hela cell lysate treated with LPS Lane2:Mouse liver tissue lysate Lane3:Rat lung tissue lysate

Anti-Claudin 5 antibody used in Western Blot (WB). GTX49370

GTX49370 WB Image

Western blot (WB) analysis of Claudin-5 pAb at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:The Liver tissue lysate of Mouse(40ug) Lane3:The Liver tissue lysate of Rat(40ug)

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf