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Claudin 8 antibody, C-term

Anti-Claudin 8 antibody, C-term used in Western Blot (WB). GTX77832
Anti-Claudin 8 antibody, C-term used in Western Blot (WB). GTX77832
Anti-Claudin 8 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX77832
Anti-Claudin 8 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX77832

Cat. No. GTX77832

Reference 10

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all Claudin 8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
ICC/IF Assay dependent
IHC-P 2-10 ug/ml
IHC Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a C-terminal region of Human Claudin 8

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_424780

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

claudin 8 , HEL-S-79

Cellular Localization

Cell membrane

Background

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This protein plays important roles in the paracellular cation barrier of the distal renal tubule, and in the paracellular barrier to prevent sodium back-leakage in distal colon. Differential expression of this gene has been observed in colorectal carcinoma and renal cell tumors, and along with claudin-7, is an immunohistochemical marker for the differential diagnosis of chromophobe renal cell carcinoma and renal oncocytoma.[provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-Claudin 8 antibody, C-term used in Western Blot (WB). GTX77832

GTX77832 WB Image

WB analysis of 293T cells using GTX77832 Claudin 8 antibody at 1.0μg/ml.

Anti-Claudin 8 antibody, C-term used in Western Blot (WB). GTX77832

GTX77832 WB Image

WB analysis of HepG2 cells using GTX77832 Claudin 8 antibody at 0.5-1.0μg/ml.

Anti-Claudin 8 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX77832

GTX77832 IHC-P Image

IHC-P analysis of human kidney tissue using GTX77832 Claudin 8 antibody at 4.0-8.0μg/ml.

Anti-Claudin 8 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX77832

GTX77832 IHC-P Image

IHC-P analysis of human intestine tissue using GTX77832 Claudin 8 antibody at 4.0-8.0μg/ml.

REFERENCE

Application Reference

Xiaojun Zhuang et al. EBioMedicine 2022; 76 : 103846 Hypermethylation of miR-145 promoter-mediated SOX9-CLDN8 pathway regulates intestinal mucosal barrier in Crohn's disease.
Application : WB, IHC-P
Reactivity : Human, Mouse
Liu C et al. Nat Commun 2019; 10 (1) : 4589 Pparg promotes differentiation and regulates mitochondrial gene expression in bladder epithelial cells.
Application : IHC-P
Reactivity : Mouse
Li L et al. Inflamm Bowel Dis 2018; (Epub) Cytokine IL9 Triggers the Pathogenesis of Inflammatory Bowel Disease Through the miR21-CLDN8 Pathway
Application : WB
Reactivity : Human
Wang H et al. Genome Biol 2016; 17 (1) : 58 Pro-inflammatory miR-223 mediates the cross-talk between the IL23 pathway and the intestinal barrier in inflammatory bowel disease.
Application : WB, ICC/IF, IHC-P
Reactivity : Human
Petersson F et al. Hum Pathol 2011; 42 (11) : 1751-60 Renal small cell oncocytoma with pseudorosettes A histomorphologic, immunohistochemical, and molecular genetic study of 10 cases.
Application : IHC
Reactivity : Human
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REVIEW

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Application Reference
Xiaojun Zhuang et al. EBioMedicine 2022; 76:103846 Hypermethylation of miR-145 promoter-mediated SOX9-CLDN8 pathway regulates intestinal mucosal barrier in Crohn's disease.
Application : WB, IHC-P
Reactivity : Human, Mouse
Liu C et al. Nat Commun 2019; 10 (1):4589 Pparg promotes differentiation and regulates mitochondrial gene expression in bladder epithelial cells.
Application : IHC-P
Reactivity : Mouse
Li L et al. Inflamm Bowel Dis 2018; (Epub) Cytokine IL9 Triggers the Pathogenesis of Inflammatory Bowel Disease Through the miR21-CLDN8 Pathway
Application : WB
Reactivity : Human
Wang H et al. Genome Biol 2016; 17 (1):58 Pro-inflammatory miR-223 mediates the cross-talk between the IL23 pathway and the intestinal barrier in inflammatory bowel disease.
Application : WB, ICC/IF, IHC-P
Reactivity : Human
Petersson F et al. Hum Pathol 2011; 42 (11):1751-60 Renal small cell oncocytoma with pseudorosettes A histomorphologic, immunohistochemical, and molecular genetic study of 10 cases.
Application : IHC
Reactivity : Human
Lechpammer M et al. Mod Pathol 2008; 21 (11):1320-9 The diagnostic and prognostic utility of claudin expression in renal cell neoplasms.
Application : IHC
Reactivity : Human
Dube E et al. Biol Reprod 2008; 78 (2):342-51 Alterations in gene expression in the caput epididymides of nonobstructive azoospermic men.
Application : IHC
Reactivity : Human
Hermo L et al. J Androl 2007; 28 (5):784-97 Structural alterations of epididymal epithelial cells in cathepsin A-deficient mice affect the blood-epididymal barrier and lead to altered sperm motility.
Application : IHC
Reactivity : Mouse
Dube E et al. Biol Reprod 2007; 76 (6):1034-44 Gene expression profiling and its relevance to the blood-epididymal barrier in the human epididymis.
Application : IHC
Reactivity : Human
Rohan S et al. Clin Cancer Res 2006; 12 (23):6937-45 Gene expression profiling separates chromophobe renal cell carcinoma from oncocytoma and identifies vesicular transport and cell junction proteins as differentially expressed genes.
Application : IHC
Reactivity : Human
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399