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Clusterin alpha antibody

Anti-Clusterin alpha antibody used in Western Blot (WB). GTX134435

Cat No. GTX134435

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Clusterin alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.
Glycerol|PBS|ProClin

Conjugation

Unconjugated

RRID

AB_2887282

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

clusterin , AAG4 , APO-J , APOJ , CLI , CLU1 , CLU2 , KUB1 , NA1/NA2 , SGP-2 , SGP2 , SP-40 , TRPM-2 , TRPM2

Cellular Localization

Cytoplasm,Cytoplasm, cytosol,Microsome,Endoplasmic reticulum

Background

The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-Clusterin alpha antibody used in Western Blot (WB). GTX134435

GTX134435 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Clusterin alpha antibody (GTX134435) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169