GeneTex
United States (US)

CoCoA antibody [N2C2], Internal

GTX105118_39771_IFA_19011506_901.jpg
GTX105118_39771_IHC_19011506_103.jpg
GTX105118_WB_19011506_405.jpg

Cat No. GTX105118

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

HeLaS3

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CoCoA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cocoa antibody, KIAA1536 antibody, calphoglin antibody, CALCOCO1 antibody, inorganic pyrophosphatase activator antibody, coiled-coil leucine zipper coactivator 1 antibody, coiled-coil coactivator protein antibody, calcium-binding and coiled-coil domain-containing protein 1 antibody, sarcoma antigen NY-SAR-3 antibody, calcium binding and coiled-coil domain 1 antibody

Cellular Localization

Cytoplasm , Nucleus

Database

Research Area

DATA IMAGES
GTX105118_39771_IFA_19011506_901.jpg

GTX105118 ICC/IF Image

CoCoA antibody [N2C2], Internal detects CALCOCO1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: CALCOCO1 protein stained by CoCoA antibody [N2C2], Internal (GTX105118) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX105118_39771_IHC_19011506_103.jpg

GTX105118 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using CoCoA(GTX105118) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105118_WB_19011506_405.jpg

GTX105118 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX105118 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf