Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using CoCoA(GTX105118) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

CoCoA antibody [N2C2], Internal detects CALCOCO1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: CALCOCO1 protein stained by CoCoA antibody [N2C2], Internal (GTX105118) diluted at 1:500.
Blue: Hoechst 33343 staining.

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX105118 diluted at 1:500