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Coagulation factor III/Tissue Factor antibody [N1C3]

KO/KD Orthogonal
Anti-Coagulation factor III/Tissue Factor antibody [N1C3] used in Western Blot (WB). GTX100808
Anti-Coagulation factor III/Tissue Factor antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX100808
Anti-Coagulation factor III/Tissue Factor antibody [N1C3] used in Western Blot (WB). GTX100808
Anti-Coagulation factor III/Tissue Factor antibody [N1C3] used in Western Blot (WB). GTX100808
Anti-Coagulation factor III/Tissue Factor antibody [N1C3] used in Western Blot (WB). GTX100808

Cat. No. GTX100808

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Coagulation factor III/Tissue Factor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Coagulation factor III/Tissue Factor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171208

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coagulation factor III, tissue factor , CD142 , TF , TFA

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Coagulation factor III/Tissue Factor antibody [N1C3] used in Western Blot (WB). GTX100808

GTX100808 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Coagulation factor III/Tissue Factor antibody [N1C3] (GTX100808) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Coagulation factor III/Tissue Factor antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX100808

GTX100808 IHC-P Image

Coagulation factor III/Tissue Factor antibody [N1C3] detects Coagulation factor III/Tissue Factor protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Coagulation factor III/Tissue Factor stained by Coagulation factor III/Tissue Factor antibody [N1C3] (GTX100808) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Coagulation factor III/Tissue Factor antibody [N1C3] used in Western Blot (WB). GTX100808

GTX100808 WB Image

Orthogonal

The data was published in the journal Diabetologia in 2015. PMID: 26271343

Anti-Coagulation factor III/Tissue Factor antibody [N1C3] used in Western Blot (WB). GTX100808

GTX100808 WB Image

Orthogonal

The data was published in the journal Diabetologia in 2015. PMID: 26271343

Anti-Coagulation factor III/Tissue Factor antibody [N1C3] used in Western Blot (WB). GTX100808

GTX100808 WB Image

KO/KD

The data was published in the journal Diabetologia in 2015. PMID: 26271343

REFERENCE

Application Reference

Ed矇n D et al. Ups J Med Sci 2019; : 1-10 Adipocytes express tissue factor and FVII and are procoagulant in a TF/FVIIa-dependent manner.
Application : WB
Reactivity : Human
Eden D et al. Diabetologia 2015; 58 (11) : 2563-72 tissue factor/factor viia signalling promotes cytokine-induced beta cell death and impairs glucose-stimulated * secretion from human pancreatic islets.
Application : WB
Reactivity : Human
Yen TY et al. J Proteomics 2014; 96 : 173-83 Using a cell line breast cancer progression system to identify biomarker candidates.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169