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Coagulation factor III/Tissue Factor antibody [N1C3]

Anti-Coagulation factor III/Tissue Factor antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX100808
Anti-Coagulation factor III/Tissue Factor antibody [N1C3] used in Western Blot (WB). GTX100808

Cat No. GTX100808

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

A431 , HeLa

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Coagulation factor III. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

coagulation factor III, tissue factor , CD142 , TF , TFA

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Coagulation factor III/Tissue Factor antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX100808

GTX100808 IHC-P Image

Coagulation factor III/Tissue Factor antibody [N1C3] detects Coagulation factor III/Tissue Factor protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Coagulation factor III/Tissue Factor stained by Coagulation factor III/Tissue Factor antibody [N1C3] (GTX100808) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Coagulation factor III/Tissue Factor antibody [N1C3] used in Western Blot (WB). GTX100808

GTX100808 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Coagulation factor III antibody [N1C3] (GTX100808) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf