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Cofilin 1 antibody [AT1C1]

Anti-Cofilin 1 antibody [AT1C1] used in Flow cytometry (FACS). GTX57545
Anti-Cofilin 1 antibody [AT1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57545
Anti-Cofilin 1 antibody [AT1C1] used in Western Blot (WB). GTX57545

Cat No. GTX57545

Host

Mouse

Clonality

Monoclonal

Clone Name

AT1C1

Isotype

IgG1

Application

WB, ICC/IF, FACS

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.4), 0.02% sodium azide, 10% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT1C1 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Cofilin 3 protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cofilin 1 , CFL , CFL1 , HELS15 , cofilin , Cofilin 1

Cellular Localization

Nucleus matrix,Cytoplasm,Cytoskeleton,Cell projection,Ruffle membrane,Lamellipodium membrane

Background

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Database

Research Area

DATA IMAGES

Anti-Cofilin 1 antibody [AT1C1] used in Flow cytometry (FACS). GTX57545

GTX57545 FACS Image

FACS analysis of HeLa cells using GTX57545 Cofilin 1 antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg

Anti-Cofilin 1 antibody [AT1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57545

GTX57545 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57545 Cofilin 1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-Cofilin 1 antibody [AT1C1] used in Western Blot (WB). GTX57545

GTX57545 WB Image

WB analysis of HeLa whole cell lysate using GTX57545 Cofilin 1 antibody.
Loading : 20 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf